|
| Status |
Public on Jul 29, 2015 |
| Title |
Rat cerebellum, PCA+NH3, sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat cerebellum after 6 months of PCA and 10 ammonia infusions
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: Cerebellum
treatment: PCA (6 months)+ Ammonium acetate (10 infusions)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not provided
|
| Label |
Biotin
|
| Label protocol |
5.5µg of ssDNA were fragmented and labeled using the WT Plus kit (Affymetrix) following protocol guides.
|
| |
|
| Hybridization protocol |
Approximately 5μg of fragmented and labeled ssDNA was hybridzed per array using the Affymetrix hybridization kit. Arrays were hybridized for 17 hours at 45° at Gene Titan platform
|
| Scan protocol |
Arrays were scanned on an Affymetrix Gene Titan platform
|
| Data processing |
RMA algorithm was used to normalize the arrays and summarized the probes.
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Jul 29, 2015 |
| Contact name |
Teresa García-Lezana |
| E-mail(s) |
teresa.garcia.lezana@vhir.org
|
| Organization name |
Vall d'Hebron Research Institute
|
| Street address |
Paseo de la Vall d'hebron 119-129
|
| City |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE71425 |
Gene expression of rat cerebellum in a new animal model of hepatic encephalopathy (HE) |
|
