|
| Status |
Public on Jun 05, 2007 |
| Title |
Melanoma_102 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Melanoma
|
| Organism |
Homo sapiens |
| Characteristics |
tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using PureGene kit following manufacturer's instructions (Gentra Systems).
|
| Label |
cy3, cy5
|
| Label protocol |
Genomic DNA Labeling Kit (Enzo Life Sciences, Farmingdale, NY) was used as per manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
Normal Human DNA
|
| Organism |
Homo sapiens |
| Characteristics |
Reference DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using PureGene kit following manufacturer's instructions (Gentra Systems).
|
| Label |
cy5, cy3
|
| Label protocol |
Genomic DNA Labeling Kit (Enzo Life Sciences, Farmingdale, NY) was used as per manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
Genomic DNA hybridization to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
|
| Scan protocol |
Scanning was performed as per manufacturer's protocol (http://www.agilent.com)
|
| Description |
expression arrays were used for CGH
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 (test/reference). Agilent software was used.
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
Sep 14, 2007 |
| Contact name |
bin feng |
| Organization name |
dfci
|
| Street address |
44 binney street
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL887 |
| Series (2) |
| GSE7606 |
Human Melanoma vs Normal Human DNA |
| GSE7615 |
Cancer Process Study |
|
