|
| Status |
Public on Jun 05, 2007 |
| Title |
Lymphoma_TKO_14 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Atm-/-,mTerc-/-,p53-/- mouse lymphoma
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6; lymphoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared by an initial extraction with Trizol (Invitrogen) according to the manufacturer’s instructions. Pelleted total RNA was then digested with RQ1 DNase (Promega) and subsequently purified through RNA purification columns (Gentra).
|
| Label |
cy3, cy5
|
| Label protocol |
Labeling to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
|
| |
|
| Channel 2 |
| Source name |
normal mouse reference
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared by an initial extraction with Trizol (Invitrogen) according to the manufacturer’s instructions. Pelleted total RNA was then digested with RQ1 DNase (Promega) and subsequently purified through RNA purification columns (Gentra).
|
| Label |
cy5, cy3
|
| Label protocol |
Labeling to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
|
| |
|
| |
| Hybridization protocol |
RNA hybridization to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
|
| Scan protocol |
Scanning was performed as per manufacturer's protocol (http://www.agilent.com)
|
| Description |
lymphoma sample from Atm-/-, mTerc-/-, p53-/- triple knock-out mice were compared to 3 week old thymus RNA from healthy mice of p53 Hets
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 (test/reference). Agilent software was used.
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
Sep 14, 2007 |
| Contact name |
bin feng |
| Organization name |
dfci
|
| Street address |
44 binney street
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL2872 |
| Series (2) |
| GSE7607 |
Atm-/-,mTerc-/-,p53-/- triple knock-out lymphoma vs normal mouse DNA (GPL2872) |
| GSE7615 |
Cancer Process Study |
|
