|
| Status |
Public on Aug 10, 2016 |
| Title |
e_iTreg_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Treg, mixed endogenous and IVIg-induced
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell type: T lymphocyte
|
| Treatment protocol |
Spleens were dissected, lymphocytes were prepared, and Treg were isolated by flow cytometric sorting.
|
| Growth protocol |
DEREG mice were sensitized and challenged with ovalbumin (OVA) to induce allergic airways disease. Endogenous Treg were depleted with injection of 40 ng/g diptheria toxin before IVIg or HSA treatment (2 g/kg).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol reagent (Life Technologies) and cleaned with RNeasy Mini silica-gel membrane columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
2 ng of total RNA were subjected to two rounds of amplification using Affymetrix GeneChip Pico WT two-cycle target labeling and control reagents according to the manufacturer's protocols
|
| |
|
| Hybridization protocol |
Following fragmentation, target ss-DNA was hybridized for 16 hours at 45°C to Affymetrix Mouse Gene 2.0 ST arrays. Arrays were stained and washed using the GeneChips Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip Scanner 3000 7G. Images were processed using GeneChip Command Console (AGCC) software to generate CEL files.
|
| Description |
Gene expression data of all Treg from allergen-exposed IVIg-treated animals
|
| Data processing |
Raw data was read using the Bioconductor oligo package. Probe intensities were normalized across all arrays by the robust multi-array average (RMA) algorithm.
|
| |
|
| Submission date |
Aug 06, 2015 |
| Last update date |
Aug 10, 2016 |
| Contact name |
Bruce David Mazer |
| E-mail(s) |
bruce.mazer@mcgill.ca
|
| Organization name |
RI-MUHC
|
| Department |
RESP
|
| Street address |
1001 boul. Décarie
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H4A 3J1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE71811 |
Gene expression data from mouse regulatory T cells |
|
