|
| Status |
Public on Dec 25, 2016 |
| Title |
probiotic strain Z21 |
| Sample type |
RNA |
| |
|
| Source name |
Zucker-Leprfa/fa male rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: intestinal mucosa
animal sex: Male rats
age: six weeks
|
| Treatment protocol |
40 Zucker-Leprfa/fa rats were randomly assigned to receive 10^10 CFUs of one of the three probiotic strains (probiotic strains groups), or a placebo (obese placebo) by oral administration each day for 30 days. An additional group of 8 Zucker-lean+/fa rats (control placebo) received placebo, and other two groups of rats (8 Zucker-lean+/fa rats and 8 Zucker-Leprfa/fa rats) were used as reference (lean reference and obese reference).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the RNeasy Mini kit and Qiacube system according to the manufacturer's instructions.
300 mg of total RNA was utilized in the cDNA synthesis with the Ambion WT Expression kit according to the manufacturer's instructions.
The resulting cDNA was fragmented using Uracil DNA Glycosylase) and APE1 (Apurinic/apyrimidinic endonuclease 1)
|
| Label |
deoxynucleotidyl transferase linked to biotin
|
| Label protocol |
The label process was performed using he WT Terminal Labeling kit de Affymetrix with deoxynucleotidyl transferase linked to biotin
|
| |
|
| Hybridization protocol |
Following fragmentation, 5,5 ug of cDNA were hybridized using the GeneChip Hybridization, Wash and Stain kit from Affymetrix
|
| Scan protocol |
GeneChips were scanned using the GeneTitan™ from Affymetrix
|
| Description |
Gene expression data from intestinal rat mucosa
|
| Data processing |
The data were analyzed with the Command Console (AGCC 3.1, Affymetrix®) and the Expression Console (EE 1.1, Affymetrix®)
|
| |
|
| Submission date |
Oct 08, 2015 |
| Last update date |
Dec 25, 2016 |
| Contact name |
Julio Ramon Plaza Diaz |
| E-mail(s) |
jrplaza@ugr.es
|
| Phone |
+34958241599
|
| Organization name |
University of Granada
|
| Department |
Biochemistry and Molecular Biology II
|
| Lab |
Biomedical Research Center
|
| Street address |
Avda. del Conocimiento s/n
|
| City |
Granada |
| State/province |
Granada |
| ZIP/Postal code |
18006 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE73848 |
Expression data from intestinal mucosa of Zucker rats |
|
