|
| Status |
Public on Oct 16, 2015 |
| Title |
Asthmatic 9 |
| Sample type |
RNA |
| |
|
| Source name |
induced sputum
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: asthmatic
gender: male
age: 42
bmi: 28.5
smoking pack-years: none
ID: FAT079
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from induced sputum using the RNeasy Mini Kit (Qiagen, Hilden, Germany);RNA quatitation was performed using the Quanti-iT RiboGreen RNA
|
| Label |
biotin
|
| Label protocol |
A total of 500 ng of sputum RNA was amplified and labelled with biotin-uridine triphosphate using the Total Prep RNA amplification kit as per manufacturer’s instructions (Life Technologies)
|
| |
|
| Hybridization protocol |
750 ng cRNA was hybridised to Illumina Sentrix HumanRef-8 V3 Expression BeadChips (Illumina, San Diego, CA, USA) using standard protocols
|
| Scan protocol |
Each BeadChip measured the expression of 24,354 genes and was scanned using the Illumina Bead Station and captured using BeadScan 3.5.11 (Illumina)
|
| Description |
postmeal
|
| Data processing |
Sample and gene profiling results were included in the analysis if the sample was of suitable purity (OD 260/280 ratio between 1.7-2.1) and was successfully amplified (sufficient cRNA generated) and hybridized (95th percentile of fluorescence score>500) and the data passed quality control criteria in GeneSpring GX (correlation coefficients and priciple component analysis plots)
|
| |
|
| Submission date |
Oct 15, 2015 |
| Last update date |
Oct 16, 2015 |
| Contact name |
QIAN LI |
| E-mail(s) |
c3156151@uon.edu.au
|
| Organization name |
University of Newcastle
|
| Lab |
HMRI
|
| Street address |
University drive
|
| City |
Newcastle |
| ZIP/Postal code |
2299 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE74075 |
Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics |
|
