|
| Status |
Public on Feb 09, 2017 |
| Title |
HSC_Mutant_RNA-Seq |
| Sample type |
SRA |
| |
|
| Source name |
dnmt3bb.1 morpholino injected embryos
|
| Organism |
Danio rerio |
| Characteristics |
strain: Ek
cell type: hematopoietic stem and progenitor cells (HSPCs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina software for base calling
reads trimmed using trimmomatc and aligned using TOPHAT
differential RNA expression calculated using cufflinks and using ANOVA from PARTEK genomic suite
Genome_build: Zv9
Supplementary_files_format_and_content: BAM
|
| |
|
| Submission date |
Nov 12, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Aniket Gore |
| Organization name |
National Institute of child health and human development
|
| Street address |
Bldg 6B center drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL14875 |
| Series (1) |
| GSE74929 |
Epigenetic regulation of hematopoiesis by DNA methylation |
|
| Relations |
| BioSample |
SAMN04261712 |
| SRA |
SRX1427534 |
