|
| Status |
Public on Jul 01, 2007 |
| Title |
Lymphoblastoid Cell Line for Individual 8091.3 log phase |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid_cell_line
|
| Organism |
Homo sapiens |
| Characteristics |
ped: 8091
id: 3
fatid: 1
motid: 2
sex: 1
country: UK
DDAST: CASE
rs3744350: CC
rs16492: TT
rs16235: GG
rs16238: CA
rs558317: TC
rs498973: CT
rs16495: CC
rs12946701: AC
rs16519: GG
rs16522: TT
rs544198: TT
rs657672: CC
rs657723: TT
rs16527: TT
rs3025203: GG
rs801259: TT
rs3744353: GG
rs2301775: AA
rs16531: TT
rs521633: AC
rs16530: CC
rs486512: CC
rs1106769: AA
rs2061342: GT
rs7214151: TC
rs7208487: TG
rs9906612: AC
rs4795369: AG
rs11655550: TT
rs4611492: TT
rs12450559: AG
rs12453682: CC
rs1877032: AA
rs879606: AA
rs9892427: TT
rs1877031: CC
rs2271308: AA
rs931992: CC
rs2941503: AA
rs2941504: AA
rs1565922: TT
rs907092: GG
rs9911688: CC
rs9303277: CC
rs2060941: GG
rs9908694: CC
rs11557467: GG
rs8067378: AA
rs2305480: CC
rs2290400: AA
rs7216389: TT
rs3744246: CC
rs4795405: CC
rs8079416: CT
rs4795408: AG
rs1007654: GG
rs3902025: AC
rs3894194: TT
rs3859192: TT
rs921651: AA
rs8066582: TT
rs8080546: CC
rs2071369: CC
rs2302776: AA
rs3934886: TT
rs11078936: TT
rs868150: TT
rs7502966: TT
rs1568400: GG
rs939348: CC
rs3744805: GG
rs2071427: GG
rs2269457: AA
rs2071570: GG
rs7211770: AA
rs2280400: CT
rs13706: GA
rs2715553: TT
rs4890109: GG
rs2715556: GG
rs2715555: CC
rs13695: GA
rs11650680: CC
rs2015561: TT
rs538399: TT
rs7214466: TT
rs8078319: CC
rs749906: CC
rs4890115: CC
rs2290207: AG
rs7214311: AG
rs896136: TT
rs9675320: TT
rs2023906: AG
rs3136685: AG
rs608517: AG
rs1358175: TC
rs757411: TC
rs11659024: AG
rs1474454: CT
rs1526603: CC
rs2014704: CA
rs2462961: TC
rs2429553: AG
rs1880832: GG
rs9915334: TC
rs9303295: AG
rs1405334: CT
rs4890126: AG
rs4890127: CC
rs2109217: CC
rs8079180: GA
rs2429542: AA
rs975078: AG
rs9898164: AG
rs719867: CC
rs981684: AG
rs12453124: CT
rs4132126: AG
rs7209228: TC
rs7219451: TC
rs7211017: GG
|
| Treatment protocol |
n/a
|
| Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
| Label |
biotin
|
| Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Description |
no additional
|
| Data processing |
RMA Express
|
| |
|
| Submission date |
Jun 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Goncalo Abecasis |
| E-mail(s) |
goncalo@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1420 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
