Fertilized 1- to 2-cell embryos were microinjected with morpholinos (MOs; 1 to 2 nL of 130 micromol/L in water; Gene Tools).
Growth protocol
Wild-type Danio rerio (zebrafish) were bred and raised following the procedures approved by the Case Western Reserve University Institutional Animal Care and Use Committee. The embryos were collected from natural spawnings, cultured, and staged as described in Cell 141:1006-1017.
Extracted molecule
total RNA
Extraction protocol
Extraction of total RNAs were performed using RNeasy Mini kit (Qiagen) per manufacturer's protocol.
Label
biotin
Label protocol
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
Hybridization protocol
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
Scan protocol
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
Data processing
The data were analyzed with Affymetrix Expression Console Software using Affymetrix default analysis settings and global scaling as normalization method.
