|
| Status |
Public on Feb 05, 2019 |
| Title |
LCAI, normoxia |
| Sample type |
RNA |
| |
|
| Source name |
normoxia
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LCAI
origin: lung cancer
|
| Treatment protocol |
The cell lines were cultured under normoxic or hypoxic condition and subjected to transcriptomic profiling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from cell cultures of myxoid liposarcoma cell line using the QIAzol reagent and the miRNeasy Mini Kit (QIAGEN) according to the manufacture’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
|
| Description |
gene expression after culture
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 or 11.5.1.1(Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Feb 05, 2016 |
| Last update date |
Feb 05, 2019 |
| Contact name |
Masatoshi Wakui |
| E-mail(s) |
wakuism@a6.keio.jp
|
| Organization name |
Keio University School of Medicine
|
| Department |
Department of Laboratory Medicine
|
| Street address |
35 Shinanomachi, Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE77606 |
Transcriptomic analysis of human tumor cell lines |
|
