|
| Status |
Public on Jul 21, 2016 |
| Title |
Before abatacept administration, Non-remission-21 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood, Rheumatoid arthritis, Before abatacept administration, Non-remission
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Whole blood
disease: Rheumatoid arthritis
sampling point: Before abatacept administration
clinical outcome: Non-remission
gender: Female
age: 64
|
| Treatment protocol |
Before administration with biologic agent, blood samples were collected in PAXgene Blood RNA tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the PAXgene Blood RNA Kit (QIAGEN) according to the manufacture's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Quick Amp Labeling Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Slides were scanned immediately after washing on an Agilent DNA Microarray Scanner using one color scan setting for 1x44k array slides.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. We applied rank-based quantile normalization to the raw signal data.
|
| |
|
| Submission date |
Feb 18, 2016 |
| Last update date |
Jul 21, 2016 |
| Contact name |
Seiji Nakamura |
| E-mail(s) |
nakamura@dna-chip.co.jp
|
| Phone |
+81-3-5777-1700
|
| Organization name |
DNA Chip Research Inc.
|
| Street address |
1-15-1, Kaigan, Suzuebaydium 5F
|
| City |
Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
105-0022 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE78068 |
Identification of baseline gene expression signatures predicting therapeutic responses to three biologic agents in rheumatoid arthritis: a retrospective observational study |
|
