|
| Status |
Public on Apr 25, 2016 |
| Title |
mouse fibrotic liver tissue, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CCl4-induced liver fibrosis at 6 weeks, biological rep2
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: Balb/c
tissue: liver
|
| Treatment protocol |
After acclimatization for one week, the hepatic fibrosis mice model was produced by administration of carbon tetrachloride (CCl4, Sigma-Aldrich, St. Louis, MO, USA) for 6 weeks, while the control Balb/c mice treated with oil for 6 weeks.
|
| Growth protocol |
All Balb/c mice aged at 8 weeks obtained from institute of laboratory animal sciences, CAMS & PUMC (Beijing , China), weighting about 20g. Mice were maintained in a 12-h light/dark cycle at 22- 25°C with free access to food and water. All protocols were approved by the Ethical Committee of Tianjin Medical University.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA ( The Ambion® WT Expression Kit For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, double-stranded cDNA were hybridized for 16 hr at 45℃ on Affymetrix Mouse Gene ST 1.0 array (Affymetrix, SantaClara, CA, USA). GeneChips were washed and stained in the GeneChip2 Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip2 Scanner 3000 7G.
|
| Description |
Gene expression data from CCl4-induced Balb/c mice liver fibrosis at 6 weeks
|
| Data processing |
Tiff images generated by the GeneChip2 Scanner 3000 7G were processed by the Genepix 6 microarray analysis software (Molecular Devices). Gene array analysis was performed according to the manufacturer’s instructions (GeneChip 430 2.0 and 230 2.0; Affymetrix, Santa Clara, CA).
|
| |
|
| Submission date |
Apr 25, 2016 |
| Last update date |
Apr 25, 2016 |
| Contact name |
Kun Zhang |
| E-mail(s) |
zhangkun@tmu.edu.cn
|
| Phone |
022-83336819
|
| Organization name |
Tianjin medical university
|
| Department |
Department of Histology and Embryology
|
| Street address |
Qixiangtai Road NO.22
|
| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE80601 |
Expression data from murine fibrotic liver tissues and normal liver tissues |
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