|
| Status |
Public on Aug 31, 2016 |
| Title |
Non-SS patient-2 |
| Sample type |
RNA |
| |
|
| Source name |
Minor salivary glands of the lower lip
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Minor salivary glands of the lower lip
Sex: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After fresh tissues were homogenized by TissueLyser, total RNA was extracted by ISOGEN.
|
| Label |
Biotin
|
| Label protocol |
10 ng of total RNA was labeled using a GeneAtlasTM 3'IVT Express Kit Assay (Affymetrix) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
7.5 µg fragmented and labeled aRNA were hybridized for 16h at 45℃ on Human Genome U219 Array Strip. Array Strip was washed and stained in the GeneAtlasTM fluidics station.
|
| Scan protocol |
Array Strip was scanned in the GeneAtlasTM imaging station.
|
| Description |
non-Sjögren’s syndrome
|
| Data processing |
The robust multichip average (RMA) method was used background correction and normalization by quantile.
|
| |
|
| Submission date |
Apr 29, 2016 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Koh-ichi Nakashiro |
| E-mail(s) |
nakako@m.ehime-u.ac.jp
|
| Phone |
+81899605393
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE80805 |
Microarray analysis of minor salivary glands from patients with primary Sjögren’s syndrome (SS) or non-SS |
|
