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Sample GSM225435 Query DataSets for GSM225435
Status Public on Jan 03, 2008
Title DDT 24h
Sample type RNA
 
Channel 1
Source name mRNA from zebrafish embryos that were exposed to DDT 24h
Organism Danio rerio
Characteristics whole body mRNA
Strains: 200-800 zebrafish from AB*,AB2O2,AB2O2 Golden and AB2O2 Leo
whole body mRNA
Strains: 200-800 zebrafish from AB*,AB2O2,AB2O2 Golden and AB2O2 Leo
Biomaterial provider Fish Facility at the Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe
Treatment protocol Acrylamide (CH2=CHCONH2), Aroclor 1254 (PCB), Arsenic(III) Oxide (As2O3), tBHQ (tert-Butylhydroquinone (CH3)3CC6H3-1,4-(OH)2), Cadmiumchloride (CdCl2.2H2O), 4-Chloroaniline (ClC6H4NH2), DDT (1,1-Bis-(4-Chlorphenyl)-2,2,2-Trichlorethan (ClC6H4)2CHCCl3), Lead (II)chloride (PbCl2), Methylmercurychloride (CH3ClHg), TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin) and Valproic acid (CH3(CH2)4CO2H) were purchased from Sigma-Aldrich. Zebrafish wild type strains AB, ABO and Tuebingen were kept and bred. Embryos were grown in embryo medium (60 ug/ml Instant Ocean, Red Sea Fish Pharm Ltd.) Different numbers of embryos were exposed to the chemicals: between 4 to 24 hpf (600 embryos), 24 to 48 hpf (400 embryos) and 96 to 120 hpf (200 embryos). Embryo medium alone (Cd, Hg, Pb, As, VA, AA) or 0.2 % ethanol in embryo medium (tBHQ , 4CA, PCB, DDT) or 0.5% DMSO, 28mg/l toluene in embryo medium (TCDD) were used as vehicle controls. The toxin concentrations were adjusted in such a way that embryo death was minimal. The few dead embryos were discarded before preparation of RNA.
Growth protocol as described in Westerfield 1993
Extracted molecule polyA RNA
Extraction protocol 1) Total RNA was isolated using the Nucleospin RNA L Kit (Macherey-Nagel, Dueren, Germany). 2) mRNA was extracted with the Ambion Purist Kit (Austin, TX).
Label Cy5
Label protocol Labeled cDNA was synthesized from 1-2 ug mRNA using the Amersham direct cDNA labeling kit (Amersham Europe, Freiburg, Germany).
 
Channel 2
Source name mRNA from zebrafish embryos that were not exposed to a toxic environment
Organism Danio rerio
Characteristics Strains: 200-800 zebrafish from AB*,AB2O2,AB2O2 Golden and AB2O2 Leo
whole body mRNA
Biomaterial provider Fish Facility at the Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe
Treatment protocol Acrylamide (CH2=CHCONH2), Aroclor 1254 (PCB), Arsenic(III) Oxide (As2O3), tBHQ (tert-Butylhydroquinone (CH3)3CC6H3-1,4-(OH)2), Cadmiumchloride (CdCl2.2H2O), 4-Chloroaniline (ClC6H4NH2), DDT (1,1-Bis-(4-Chlorphenyl)-2,2,2-Trichlorethan (ClC6H4)2CHCCl3), Lead (II)chloride (PbCl2), Methylmercurychloride (CH3ClHg), TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin) and Valproic acid (CH3(CH2)4CO2H) were purchased from Sigma-Aldrich. Zebrafish wild type strains AB, ABO and Tuebingen were kept and bred. Embryos were grown in embryo medium (60 ug/ml Instant Ocean, Red Sea Fish Pharm Ltd.) Different numbers of embryos were exposed to the chemicals: between 4 to 24 hpf (600 embryos), 24 to 48 hpf (400 embryos) and 96 to 120 hpf (200 embryos). Embryo medium alone (Cd, Hg, Pb, As, VA, AA) or 0.2 % ethanol in embryo medium (tBHQ , 4CA, PCB, DDT) or 0.5% DMSO, 28mg/l toluene in embryo medium (TCDD) were used as vehicle controls. The toxin concentrations were adjusted in such a way that embryo death was minimal. The few dead embryos were discarded before preparation of RNA.
Growth protocol as described in Westerfield 1993
Extracted molecule polyA RNA
Extraction protocol 1) Total RNA was isolated using the Nucleospin RNA L Kit (Macherey-Nagel, Dueren, Germany).
2) mRNA was extracted with the Ambion Purist Kit (Austin, TX).
Label Cy3
Label protocol Labeled cDNA was synthesized from 1-2 ug mRNA using the Amersham direct cDNA labeling kit (Amersham Europe, Freiburg, Germany).
 
 
Hybridization protocol 1) Removal of unincorporated nucleotides over Microcon 30 spin columns (Millipore, Bedford, MA).
2) The concentrated probes were hybridized to the microarray in 1xDIG Easy-Hyb buffer (Hoffmann-La Roche, Basel, CH) overnight at 42 C.
3) Cover slips were removed from the slides by flushing with 4xSSC.
4) Slides were washed in pre-warmed wash buffer 1 (2xSSC, 0.1% SDS) for 5 minutes at 42 C.
5) Washing in buffer 2 (0.1xSSC, 0.1% SDS) for 10 minutes at room temperature.
6) Washing in 0.1xSSC four times for 1 min at room temperature.
7) The slides were briefly dipped into 0.01xSSC at room temperature.
8) Centrifugation for 7 min at 800 rpm in an Eppendorf 5810R centrifuge.
Scan protocol 1) Scanned on an GenePix 4000B dual-laser scanner from Molecular Devices
2) Both channels (532 nm for Cy3 and 635 nm for Cy5) were scanned in parallel
3) Data was stored as 16-bit TIFF files
4) The channels for Cy3 and Cy5 were balanced in each scan for approximately the same intensity profile.
5) Each array was scanned three times (low, medium and high scan) with different signal amplification factors (voltage settings of the photomultiplier tubes), but with the same laser power.
6) Raw data was retrieved with GenePix Pro 6.0 software from Axon Instruments .
7) Data processing was performed
8) The absolute intensity values span the range from 0 to 65536.
9) The scans were performed with a resolution of 10 um.
Description This file contains all technological and biological replicates (i.e. from the same and different biological source, repspectively) that were performed with this particular toxin at this particular concentration. The Dye-Swap Designe was utilised.
Data processing 1) Raw data are retrieved with GenePix 2) Quality control on spot level 3) Local background correction. 4) Averaging of the two spots for each gene. 5) Consensus signals are calculated from low, medium and high scan based on a Least Squares Algorithm. 6) Log transformation. 7) locally weighted regression smoother (LOESS). 8) Scaling to a common MAD. 9) t-test 10) adjustment of p-values for multiplicity 11) Quality control on array level.
 
Submission date Aug 29, 2007
Last update date Jan 02, 2008
Contact name Jessica Legradi
Organization name Karlsruhe Institute of Technology
Department Institute of Toxicology and Genomics ITG
Lab Straehle
Street address Hermann-von-Helmholtz-Platz 1
City Eggenstein-Leopoldshafen
State/province Baden-Wuerttemberg
ZIP/Postal code 76344
Country Germany
 
Platform ID GPL4603
Series (1)
GSE9357 biosensor-series

Data table header descriptions
ID_REF spot/gene id (numbered from 1-34992)
VALUE normalized log naturalis ratios (M or M.bar value) (test/reference)
res_A.bar averaged log naturalis average
res_M.var variance of VALUE
res_M.se standard error of VALUE
res_par.state1 Check for reliability of p-value and fold change: A.bar + 0.5 * M.bar
res_par.state2 Check for reliability of p-value and fold change: A.bar - 0.5 * M.bar
res_t test statistic of t-test for this gene
res_t.reg test statistic of regularized t-test for this gene
res_fc fold change
res_p.value p-value for t-test
res_p.adj adjusted p-value for t-test
res_wilcox.stat test statistic for wilcoxon test
res_p.value.adj.wil adjusted p-value for wilcoxon test
res_good.arrays numnber of good flags (after quality control) for this gene
res_comment1 Comment if FC is not reliable
res_comment2 Comment if t and regularized t-values are not reliable
NA.
norm_flag_Array1 flag if spot is good (1) or bad (0)
norm_flag_Array2
norm_flag_Array3
norm_flag_Array4
norm_flag_Array5
norm_flag_Array6
norm_R_Array1 Red signal after normalization
norm_R_Array2
norm_R_Array3
norm_R_Array4
norm_R_Array5
norm_R_Array6
norm_G_Array1 Green signal after normalization
norm_G_Array2
norm_G_Array3
norm_G_Array4
norm_G_Array5
norm_G_Array6
norm_M_Array1 M value after normalization
norm_M_Array2
norm_M_Array3
norm_M_Array4
norm_M_Array5
norm_M_Array6
norm_A_Array1 A value after normalization
norm_A_Array2
norm_A_Array3
norm_A_Array4
norm_A_Array5
norm_A_Array6
norm_C_Array1 Normalization constant that will be added to signal
norm_C_Array2
norm_C_Array3
norm_C_Array4
norm_C_Array5
norm_C_Array6
avg_R_Array1 Red channel value after averaging of scans
avg_R_Array2
avg_R_Array3
avg_R_Array4
avg_R_Array5
avg_R_Array6
avg_G_Array1 Green channel value after averaging of scans
avg_G_Array2
avg_G_Array3
avg_G_Array4
avg_G_Array5
avg_G_Array6
avg_flag_Array1 Flag (good/bad spot) value after averaging of scans
avg_flag_Array2
avg_flag_Array3
avg_flag_Array4
avg_flag_Array5
avg_flag_Array6

Data table
ID_REF VALUE res_A.bar res_M.var res_M.se res_par.state1 res_par.state2 res_t res_t.reg res_fc res_p.value res_p.adj res_wilcox.stat res_p.value.adj.wil res_good.arrays res_comment1 res_comment2 NA. norm_flag_Array1 norm_flag_Array2 norm_flag_Array3 norm_flag_Array4 norm_flag_Array5 norm_flag_Array6 norm_R_Array1 norm_R_Array2 norm_R_Array3 norm_R_Array4 norm_R_Array5 norm_R_Array6 norm_G_Array1 norm_G_Array2 norm_G_Array3 norm_G_Array4 norm_G_Array5 norm_G_Array6 norm_M_Array1 norm_M_Array2 norm_M_Array3 norm_M_Array4 norm_M_Array5 norm_M_Array6 norm_A_Array1 norm_A_Array2 norm_A_Array3 norm_A_Array4 norm_A_Array5 norm_A_Array6 norm_C_Array1 norm_C_Array2 norm_C_Array3 norm_C_Array4 norm_C_Array5 norm_C_Array6 avg_R_Array1 avg_R_Array2 avg_R_Array3 avg_R_Array4 avg_R_Array5 avg_R_Array6 avg_G_Array1 avg_G_Array2 avg_G_Array3 avg_G_Array4 avg_G_Array5 avg_G_Array6 avg_flag_Array1 avg_flag_Array2 avg_flag_Array3 avg_flag_Array4 avg_flag_Array5 avg_flag_Array6
1 0.0686285782766941 6.41772131038315 0.0883262385309025 0.154951930027075 6.4520355995215 6.3834070212448 0.516351135320315 0.442902377948456 1.07103832836049 0.680734227088863 0.844190311415589 9 0.997199062011464 5 TRUE FALSE TRUE TRUE TRUE FALSE 6.59415116675604 5.82633333155625 5.38572384703869 6.67096336352602 6.5634745216651 5.98620996160015 6.01925999630601 6.7554105818063 0.0306766450909395 -0.159876630043904 -0.633536149267318 -0.0844472182802776 6.57881284421057 5.9062716465782 5.70249192167235 6.71318697266616 -0.479674684418726 -0.342224316702799 -0.215218375616351 -0.325158740461724 574.967676564422 285.779682545472 196 670.741618353273 900.825769093904 472.161987210851 458 1010.28722645724 TRUE FALSE TRUE TRUE TRUE FALSE
2 0.0863438926094412 7.00280300481612 0.0295603070755747 0.105924707002232 7.04597495112084 6.9596310585114 1.23013500899744 0.81514403063321 1.09018116909595 0.45205487878353 0.674964711593607 14 0.77872289919291 6 TRUE TRUE TRUE TRUE TRUE TRUE 6.86533130556726 7.01913925750317 6.56558758438356 6.85740902685153 7.55349255856584 6.81881155415557 6.90032735801524 7.03778307056349 6.5687141743278 6.92981987884827 7.56411179284407 7.23039817712807 -0.0349960524479793 -0.0186438130603152 -0.00312658994424236 -0.0724108519967382 -0.0106192342782395 -0.411586622972508 6.88282933179125 7.02846116403333 6.56715087935568 6.8936144528499 7.55880217570495 7.02460486564182 -0.482045638118292 -0.480250623150813 -0.341557899846035 -0.264849645637444 -0.232315511559429 0.576713064381081 753.183196088407 879.201356464526 598.728736211667 832.958819052312 1698.2180981889 1220.68576998064 1263.13269108607 1447.95628437171 845.132250223235 1167.06193361034 2165.20332077188 1034.88091896230 TRUE TRUE TRUE TRUE TRUE TRUE
3 0.03332783288284 6.85728429231853 0.130762822937021 0.146453873705355 6.87394820875995 6.84062037587711 0.225756652237317 0.227565389973167 1.03388942664033 0.828996762873889 0.921774856259394 11 1 6 TRUE TRUE TRUE TRUE TRUE TRUE 7.2431241275162 7.19687672474288 6.25907443788814 6.472965963158 6.83660667261266 5.85688819097805 7.07626936168675 7.01045004335493 6.39638548236575 6.52540581843979 7.31489997897943 6.7069640420102 0.166854765829444 0.186426681387956 -0.137311044477611 -0.0524398552817822 -0.47829330636677 -0.850075851032146 7.15969674460147 7.1036633840489 6.32772996012694 6.4991858907989 7.07575332579604 6.28192611649413 -0.499684526864037 -0.480423051053653 -0.335713264841028 -0.250412614247468 -0.271984402335658 0.686145803191277 1089.29051985875 1050.12575139731 441.95930401195 571.211564066138 812.905178752269 492.729869869205 1519.46203236610 1409.03671476749 709.274144059204 773.2575113857 1721.39825782683 580.500777450596 TRUE TRUE TRUE TRUE TRUE TRUE
4 -0.111568624001302 8.3156972074706 0.0129655009020563 0.080023156649468 8.25991289546995 8.37148151947125 -2.40006526804959 -1.39420423628145 -1.11803046509714 0.222040981803055 0.406240334935574 2 0.191640378548896 6 TRUE TRUE TRUE TRUE TRUE TRUE 8.47018171599349 8.75328155361902 7.6377386347859 8.1011411885191 8.38425908008901 7.97107072406346 8.90943074458457 8.67384744724657 7.89995129540054 8.13134290757495 8.68234726741828 8.01594212931108 -0.439249028591081 0.0794341063724499 -0.262212660614637 -0.0302017190558362 -0.298088187329263 -0.0448714052476193 8.68980623028903 8.7135645004328 7.76884496509322 8.11624204804703 8.53330317375365 7.99350642668727 -0.495071973858132 -0.542436889607485 -0.321022808649661 -0.274286129568631 -0.154028482644037 0.483442125558475 3724.34296143716 4827.39907981452 1767.33356711658 2875.54679218733 4053.13055790086 3687.82372580181 9480.25725527855 7669.95396241291 3166.75505211546 3899.04126480397 6370.04306245449 2378.48989296512 TRUE TRUE TRUE TRUE TRUE TRUE
5 -0.0237551929337440 6.40578040712566 0.0666534979906416 0.149126225188555 6.39390281065879 6.41765800359253 -0.205746327078244 -0.159295877728468 -1.02403959507352 0.881155500772648 0.945639158507423 7 1 5 TRUE TRUE FALSE TRUE TRUE FALSE 7.09901525948846 6.68110049463947 5.85401775664764 6.34736034642676 7.05474137154357 6.82593982399197 6.35833015813013 6.5048043144697 0.044273887944884 -0.144839329352506 -0.504312401482494 -0.157443968042929 7.07687831551601 6.75352015931572 6.10617395738889 6.42608233044823 -0.49320140024912 -0.491073541248775 -0.215629132214972 -0.357591545270084 946.162716827628 623.634041277191 313 477.500197623413 1482.28792990010 1177.88601093231 643 799.191096778088 TRUE TRUE FALSE TRUE TRUE FALSE
6 -0.168860666254622 7.24791808645624 0.141907119069222 0.142631905832883 7.16348775332893 7.33234841958355 -1.09799922108841 -1.18389125678844 -1.18395516252369 0.289666492924892 0.498524981331292 5 0.521260368004576 6 TRUE TRUE TRUE TRUE TRUE FALSE 7.62037528715031 6.96548882313705 5.76448667459489 6.44728869085691 6.61404364114513 7.81098667009946 7.29127392192124 6.41133036432822 6.67893650801634 7.01812379417558 -0.190611382949147 -0.325785098784186 -0.646843689733324 -0.231647817159434 -0.404080153030443 7.71568097862489 7.12838137252914 6.08790851946155 6.56311259943663 6.81608371766036 -0.520528290510893 -0.479773183955018 -0.33806968399282 -0.256408131525173 -0.309722754825639 1572.01126956018 833.473769308162 269.199255616702 555.064667565257 638.538686775802 3201.10331517478 1865.26891848196 720.802522826398 904.281380684071 1303.73065936867 TRUE TRUE TRUE TRUE TRUE FALSE
7 0.0858901831281387 6.47990095513402 0.195267684472405 0.180981507114657 6.5228460466981 6.43695586356995 0.434623637987263 0.474579886627449 1.08968665575437 0.659835075771828 0.830239085631349 8 1 5 TRUE TRUE FALSE TRUE TRUE FALSE 8.42687766122991 6.61647594675105 5.81182949284355 6.12103516159174 6.96732064038074 6.66980108170624 6.14348771146926 6.68368247567353 1.45955702084917 -0.0533251349551856 -0.331658218625718 -0.562647314081791 7.69709915080533 6.64313851422864 5.97765860215640 6.40235881863263 -0.521028827284558 -0.503714395954902 -0.210027034554268 -0.360345962784055 3520.51740408697 580.92330836857 300.911431989021 380.262643165367 1377.23762914184 1014.00009687002 517.237023457233 957.049828088412 TRUE TRUE FALSE TRUE TRUE FALSE
8 -0.0962085187476295 8.61319884149599 0.149426138207616 0.137571078113538 8.56509458212217 8.6613031008698 -0.609643386768689 -0.699336808774792 -1.10098861682765 0.515529405466189 0.729306449164 8 0.868358359803639 6 TRUE TRUE TRUE TRUE TRUE TRUE 9.0153731629365 9.11171333773807 7.49745235885126 8.00634678019649 8.38932424765792 7.78069315151237 9.44487430989158 8.96637109774868 7.98502922342594 8.24173317767609 8.91538412141638 8.4305929803443 -0.429501146955083 0.145342239989383 -0.487576864574682 -0.235386397479598 -0.526059873758451 -0.649899828831927 9.23012373641403 9.03904221774337 7.7412407911386 8.12403997893629 8.65235418453715 8.10564306592833 -0.465130776141429 -0.565150631431705 -0.321971159800231 -0.274198003095442 -0.148910392664285 0.482022844579793 6521.16144547716 6830.3966259097 1535.27804273595 2615.59733983584 4084.1505881926 3046.36566484426 15953.5985623855 10393.6093789528 3449.60428458327 4353.92135627750 8021.1679324747 3603.2141950112 TRUE TRUE TRUE TRUE TRUE TRUE
9 -0.190013535002007 9.07689678835894 0.136804206918440 0.134277624178149 8.98189002085793 9.17190355585994 -1.25837602810506 -1.41507966174552 -1.20926596496375 0.216197721562437 0.397331442905083 6 0.671270718232044 6 TRUE TRUE TRUE TRUE TRUE TRUE 9.42216701076446 10.2563376123599 7.69711661467766 8.34753118897973 8.9940055340869 9.15874608874006 9.26094503108068 10.5057074379481 8.3066414044405 8.1905422954256 9.71547105017493 9.13634378837129 0.161221979683788 -0.24936982558819 -0.609524789762837 0.156988893554128 -0.72146551608804 0.0224023003687733 9.34155602092257 10.381022525154 8.00187900955908 8.26903674220267 9.35473829213091 9.14754493855568 -0.45846229168553 -0.595727437755929 -0.313276382645745 -0.273357664715723 -0.119175176477712 0.439919713026707 9827.46076501973 21130.5236685709 1882.73055199583 3680.67838020817 7588.71797101509 11833.6913971704 13229.1257880294 49196.1750624945 4737.56986145572 4134.91118250053 17589.5215705061 7453.08956306206 TRUE TRUE TRUE TRUE TRUE TRUE
10 0.0234616084963510 7.47788401086899 0.0961374508732559 0.12375801499417 7.48961481511716 7.46615320662081 0.185347659980498 0.189576477107008 1.02373899711273 0.857096694277139 0.933471333723996 12 0.997199062011464 6 TRUE TRUE TRUE TRUE TRUE TRUE 8.33413333502976 8.19137400791493 6.28625018915462 6.66960018623437 7.30903330606051 6.6293474456396 8.39175239130439 8.23331630082119 6.73765172264357 7.18115928351686 7.64878553026578 7.18376156574943 -0.0576190562746300 -0.0419422929062611 -0.451401533488954 -0.511559097282492 -0.339752224205265 -0.554414120109824 8.36294286316708 8.21234515436806 6.5119509558991 6.92537973487561 7.47890941816314 6.90655450569452 -0.502349621639889 -0.5203258472076 -0.339771556108882 -0.264759568440915 -0.238620581580528 0.594632260363418 3238.80114782399 2782.79496386466 453.213995914185 690.364971542375 1325.73594613417 1019.08819072403 5669.90250747058 4882.82499819017 999.782245427847 1500.47809714663 2363.96558639153 978.915607021112 TRUE TRUE TRUE TRUE TRUE TRUE
11 0 FC is not reliable t and regul. t values unreliable t and regul. t values unreliable FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE FALSE
12 -0.0375116535541311 7.89224904462375 0.176018457051181 0.147959197441346 7.87349321784668 7.91100487140081 -0.219009386729012 -0.253527014222968 -1.03822409601203 0.809955684865225 0.912197274695975 9 0.997199062011464 6 TRUE TRUE TRUE FALSE TRUE TRUE 8.50744197446342 8.20863038385383 6.79439964524817 7.88373542746343 6.97355410271978 8.64659845662247 8.12126038014117 7.29042642901808 8.35900692576937 7.85858009272694 -0.139156482159047 0.0873700037126582 -0.496026783769911 -0.475271498305944 -0.885025990007165 8.57702021554295 8.1649453819975 7.04241303713313 8.1213711766164 7.41606709772336 -0.496109578887387 -0.517341945325458 -0.335316154519119 -0.183820171949893 0.532708496797244 3863.72562589362 2835.45986020043 755.017983362558 2420.73221480744 1393.96620956786 7292.87589667245 4358.70970273197 1733.82374601538 4679.36179837575 1982.72183595977 TRUE TRUE TRUE FALSE TRUE TRUE
13 -0.0778746084950755 11.177775513627 0.0210525424494662 0.100757636216446 11.1388382093795 11.2167128178745 -1.31467748596297 -0.772890387461911 -1.08098710357181 0.474519651738292 0.694788937499932 5 0.521260368004576 6 TRUE TRUE TRUE TRUE TRUE TRUE 11.4307869913216 12.1647086130602 10.1454138325154 10.5280431582417 11.1683775854489 10.8925453780148 11.6716141015938 11.9277233100675 10.2343637523716 10.4060903835068 11.4058070022519 11.1576278250039 -0.240827110272239 0.236985302992701 -0.0889499198562695 0.121952774734921 -0.237429416802991 -0.265082446989145 11.5512005464577 12.0462159615639 10.1898887924435 10.4670667708743 11.2870922938504 11.0250866015094 -0.394986693360805 -0.573075393493433 -0.300275351544861 -0.189084433366825 -0.0920676868565224 0.481752395628667 75606.2056896843 144086.762764394 21922.6615933036 33979.1011162648 67666.3129512433 68420.076445539 142786.737264338 201644.395521174 32354.2746301495 36338.4831361029 94074.363134961 55091.5500738677 TRUE TRUE TRUE TRUE TRUE TRUE
14 -0.0302347914551454 8.70465829081525 0.070215832743044 0.107796842140041 8.68954089508767 8.71977568654282 -0.279489229988112 -0.28047938005333 -1.03069650427818 0.790344608298725 0.902626534853216 9 0.997199062011464 6 TRUE TRUE TRUE TRUE TRUE TRUE 9.18813895249649 9.55295712655404 7.48824475430705 7.55214800086936 8.96251811669473 8.204136963767 9.32155361281497 9.35488082240838 7.7161354692903 7.80200430891296 9.39840224238079 8.7861192143764 -0.133414660318480 0.198076304145656 -0.227890714983253 -0.249856308043597 -0.435884125686058 -0.581982250609403 9.25484628265573 9.45391897448121 7.60219011179868 7.67707615489116 9.18046017953776 8.4951280890717 -0.46347443116979 -0.576181845452816 -0.326925408358273 -0.277809077006394 -0.128257462943576 0.467627686889141 7757.39403784331 10560.3975552348 1517.44313608273 1657.79463074054 7320.17405898812 4619.0798945222 14090.9894839577 15413.0794848621 2642.81039498192 2809.91313044109 12868.4128827561 5178.67945316689 TRUE TRUE TRUE TRUE TRUE TRUE
15 0.150005521889672 7.17953397657839 0.0553407794367099 0.112390515351271 7.25453673752322 7.10453121563355 1.56192521375808 1.33468132449465 1.16184065826650 0.239528479911753 0.431239996350692 16 0.521260368004576 6 FALSE TRUE TRUE TRUE TRUE TRUE 8.15012632081705 6.3307579676182 6.31785977112605 7.22264605207749 6.59062984770802 8.21909330548846 6.36930264033509 6.58681142971275 7.48587210528032 7.25062311884685 -0.0689669846714043 -0.0385446727168972 -0.268951658586698 -0.263226053202831 -0.659993271138827 8.18460981315276 6.35003030397664 6.4523356004194 7.3542590786789 6.92062648327744 -0.518603027891598 -0.335791630815196 -0.246088621334854 -0.248112672925647 0.591889206680116 2672.64742978169 474.785039038485 490.200975187504 1210.25906092740 979.041910435758 4809.72322293218 690.34982077172 820.452272780115 2018.13367842154 1048.04155467967 FALSE TRUE TRUE TRUE TRUE TRUE
16 -0.040262322768767 9.0346002335007 0.125910925577279 0.130381502091914 9.01446907211631 9.05473139488508 -0.277934681740271 -0.308803949354591 -1.04108383837035 0.769923237287002 0.892091189375721 9 0.997199062011464 6 TRUE TRUE TRUE TRUE TRUE TRUE 9.9104004243853 9.7168497717256 7.99900742515035 8.18696237810172 9.06864452267818 8.21412531456287 10.0100394825523 9.61931396098431 8.36123993865767 8.45040152156463 9.54583004532405 8.8360149931777 -0.0996390581670238 0.0975358107412876 -0.362232513507324 -0.263439143462917 -0.477185522645876 -0.621889678614833 9.9602199534688 9.66808186635496 8.180123681904 8.31868194983317 9.30723728400112 8.52507015387029 -0.424387341650221 -0.581664767294693 -0.306083792554147 -0.273180409179211 -0.122087318231492 0.466189569362221 16288.3719278577 12407.0158764464 2555.40378893541 3134.96150383171 8164.90902736578 4662.09454467896 27508.0275888240 20133.6202228315 4985.46438703502 5361.46064938319 14866.6199125565 5447.54435197048 TRUE TRUE TRUE TRUE TRUE TRUE
17 0.132600600712136 6.71331316386138 0.108647064208694 0.143387450113940 6.77961346421744 6.64701286350531 0.985399003898884 0.924771314412574 1.14179387558861 0.397522464892342 0.622153416741785 14 0.77872289919291 6 TRUE TRUE TRUE TRUE TRUE TRUE 6.92377918535111 6.92795387273153 5.78851917552388 6.50668261524035 7.42879059057075 6.22323454662504 6.32424832859248 6.95765053204828 6.11262676827279 6.48556919157412 7.46301656433292 6.88953408385793 0.599530856758634 -0.0296966593167531 -0.324107592748903 0.0211134236662340 -0.0342259737621706 -0.666299537232887 6.6240137569718 6.9428022023899 5.95057297189833 6.49612590340723 7.44590357745183 6.55638431524148 -0.478777830443578 -0.481213182639082 -0.341258615399964 -0.250125230505725 -0.241547933338385 0.646656526840636 799.822853585045 802.191439028277 275.307833958881 590.884165058805 1492.21462939778 696.844864568797 708.845254442792 1337.09833497352 535.529552963947 742.952272780115 1966.06729791177 710.668318129986 TRUE TRUE TRUE TRUE TRUE TRUE
18 0.0471581649586912 7.57932992310477 0.0411419526072037 0.0997144389468076 7.60290900558412 7.55575084062543 0.56949520617537 0.472932159642874 1.04828779836461 0.656186687551068 0.827961729762552 12 0.997199062011464 6 TRUE TRUE TRUE TRUE TRUE TRUE 8.61895451939706 8.63764105831067 6.62592101334797 6.73960414306927 7.82014813908844 6.49152308049377 8.56142675436643 8.5509045076896 6.78823716218687 6.85430689692883 7.81436968033137 7.15565333088705 0.0575277650306243 0.0867365506210678 -0.162316148838897 -0.114702753859559 0.00577845875707034 -0.664130250393288 8.59019063688174 8.59427278300013 6.70707908776742 6.79695551999905 7.8172589097099 6.82358820569041 -0.496038859754011 -0.536018049839344 -0.341927038759697 -0.264293526099687 -0.21115700446054 0.610429318022837 4319.67345611994 4314.04834486121 635.846698358315 740.597564570466 2240.75835044088 894.923052309125 6697.20921978072 6760.91511968402 1052.79170501691 1081.87795790244 2751.62845969772 944.295051509489 TRUE TRUE TRUE TRUE TRUE TRUE
19 0.0185141987788569 5.92619434832504 0.0237789024873909 0.162576028021740 5.93545144771447 5.91693724893561 0.268468923542529 0.113880250392027 1.01868664917335 0.914819791860078 0.959813076537802 9 0.997199062011464 5 TRUE FALSE FALSE TRUE TRUE FALSE 6.11794624156187 5.51749072959667 6.11290469240913 6.33046543025672 5.47243531067586 5.99209522913145 -0.212519188694851 0.0450554189208013 0.120809463277687 6.22420583590929 5.49496302013626 6.05249996077029 -0.491294510918123 -0.229626695448772 -0.417630921493935 355.064330630793 222 366.533246845840 717.743803486155 267 493.197987051821 TRUE FALSE FALSE TRUE TRUE FALSE
20 -0.0475596576445249 8.52547713724608 0.0191459715226947 0.0828860696957187 8.50169730842382 8.54925696606834 -0.841929332806868 -0.573795546333904 -1.04870876274971 0.59095074962876 0.783753359271132 6 0.671270718232044 6 FALSE FALSE TRUE TRUE TRUE TRUE 7.50285997520402 7.75055256379972 8.52736574139129 7.86359812479732 7.71743764465931 7.76188081451292 8.8382757239695 8.21305480785348 -0.214577669455288 -0.0113282507132013 -0.310909982578204 -0.349456683056159 7.61014880993166 7.75621668915632 8.6828207326804 8.0383264663254 -0.326641131820628 -0.276706274759502 -0.147659510684875 0.482553977328374 1540.00263938675 2022.72203715522 4691.63298863928 3310.56819401169 2645.8779319732 2697.91343639586 7421.26999084877 2898.00491590668 FALSE FALSE TRUE TRUE TRUE TRUE

Total number of rows: 34992

Table truncated, full table size 18650 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
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