|
| Status |
Public on Jan 20, 2017 |
| Title |
epidermis_D51_old_65y |
| Sample type |
RNA |
| |
|
| Source name |
human epidermis, inner forearm
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: epidermis
gender: female
age: 65
group: old
|
| Treatment protocol |
no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Suction blisters were obtained as described previously from the inner forearms of 28 young (20 to 25 years) and 54 old (aged between 55 and 66 years) healthy female volunteers (Sudel et al Photochem Photobiol 2003). Suction blister roofs were taken and immediately stored at -80°C . For transcriptomics analysis the suction blister epidermis samples were homogenized using a ball mill. RNA was extracted using RNeasy Fibrous Tissue Mini Kit (Qiagen) according to manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent Whole Human Genome Oligo Microarrays 8x60K V2 Kit
|
| |
|
| Hybridization protocol |
done by Genomics Services from Miltenyi Biotec
|
| Scan protocol |
done by Genomics Services from Miltenyi Biotec
|
| Description |
epidermis_D51_old_65y
|
| Data processing |
Agilent Feature Extraction Software; data processing using LIMMA for R
|
| |
|
| Submission date |
Aug 09, 2016 |
| Last update date |
Jan 20, 2017 |
| Contact name |
Nicola Zamboni |
| Organization name |
ETH Zurich
|
| Department |
Institute for Molecular Systems Biology
|
| Lab |
Zamboni Lab
|
| Street address |
Auguste-Piccard-Hof 1
|
| City |
Zürich |
| State/province |
Zurich |
| ZIP/Postal code |
8049 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE85358 |
Metabolic alterations in aged human skin in vivo |
|
