|
| Status |
Public on Oct 05, 2007 |
| Title |
Stroma normal breast (NS3) |
| Sample type |
RNA |
| |
|
| Source name |
stroma
|
| Organism |
Homo sapiens |
| Characteristics |
normal
|
| Treatment protocol |
RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
|
| Growth protocol |
RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
|
| Label |
biotin
|
| Label protocol |
Biotynylated cRNA were prepared according to the standard affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16hr at 45c on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the affymetrix fludics station 400
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanncer G2500A
|
| Description |
expression of breast tissue
|
| Data processing |
dCHIP with default settings
|
| |
|
| Submission date |
Sep 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Andrea Richardson |
| E-mail(s) |
arichardson@partners.org
|
| Phone |
617-732-4764
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Pathology
|
| Street address |
75 Francis Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8977 |
Bone-marrow-derived mesenchymal stem cells promote breast cancer metastasis |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
