|
| Status |
Public on Jun 08, 2017 |
| Title |
Bcell_Donor5-NS-2 |
| Sample type |
RNA |
| |
|
| Source name |
Naïve B cells purified from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
donor: 5
cell type: naive B cells
treatment: mock treated
replicsate: 2
|
| Treatment protocol |
Naïve B cells were cultured with sCD40L at 2.5ug/ml for 6 hours.
|
| Growth protocol |
Naïve B cells were purified from healthy donor PBMC using negative selection beads (Miltenyi)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cell cultures with the Qiagen RNeasy Kit in 96-well plates.
|
| Label |
biotin
|
| Label protocol |
Subject cDNA was amplified through utilization of the NuGEN Ovation Pico WTA System V2 (NuGEN, San Carlos, CA, USA).
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed on GeneChip Human Genome U133 Plus 2.0 Array according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA).
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
Data were normalized using Robust Multi-array Average (RMA) algorithm and log base 2 transformed using R/Bioconductor.
|
| |
|
| Submission date |
Aug 12, 2016 |
| Last update date |
Jun 09, 2017 |
| Contact name |
Alice Walsh |
| E-mail(s) |
awalsh17@its.jnj.com
|
| Organization name |
Janssen R&D
|
| Department |
Immunology
|
| Street address |
Welsh & McKean Roads
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE85543 |
Transcriptional effects of soluble CD40 ligand on human naïve B cells |
|
