|
| Status |
Public on Aug 24, 2016 |
| Title |
XL40_2 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampal neurons_XL40
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
tissue/cell type: primary rat hippocampal neurons
treated with: XL40; cross-linked Ab40 oligomers
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 23, 2016 |
| Last update date |
Aug 24, 2016 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE85952 |
Design, characterization, and use of a novel amyloid β-protein control for assembly, neurotoxicity, and gene expression studies |
|
