|
| Status |
Public on Apr 18, 2019 |
| Title |
6h embryo RNAseq (replicate 1) |
| Sample type |
SRA |
| |
|
| Source name |
w1118 6h RNAseq
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
genotype/variation: wild type
tissue: embryo
age: 6h
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNAs were isolated from embryos at indicated stages using TRIzol reagent (Invitrogen) and mRNAs were then purified using a Dynabeads mRNA DIRECT Kit (Invitrogen) according to the user manual.
Illumina sequencing libraries were constructed according to the manufacturer’s instructions. The libraries were then sequenced using the Illumina Hiseq2500 V4 platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
processed data file: 6h_FPKM.txt
|
| Data processing |
FastQC was used to make sure that the sequencing data were high quality
Reads were aligned to the dm6 genome assembly using STAR or Tophat2
Differential expression analysis was carried out by cuffdiff
Genome_build: dm6
|
| |
|
| Submission date |
Sep 15, 2016 |
| Last update date |
Apr 18, 2019 |
| Contact name |
Shunmin He |
| E-mail(s) |
hesm@ioz.ac.cn
|
| Phone |
010-64807279
|
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE86795 |
6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
|
| Relations |
| BioSample |
SAMN05773556 |
| SRA |
SRX2165462 |
