|
| Status |
Public on May 23, 2018 |
| Title |
Dicer KO BMDMs, Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Dicer knockout bone marrow-derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
cell type: macrophages
genotype: LysM-Cre/Dicerflox/flox/Apoe–/–
age: 6-8 weeks
Sex: Male
|
| Growth protocol |
mouse bone marrow-derived macrophages were cultured in DMEM-F12/10% FCS/10% L929-conditioned medium, at 37°C in a humidified atmosphere of 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana miRNA Isolation Kit, see manufacturer's web site
|
| Label |
FAM
|
| Label protocol |
cDNA was obtained by TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) and amplified by TaqMan PreAmp Master Mix (Applied Biosystems)
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Biological replicate 2. of 3 BMDMs from LysM-Cre/Dicerflox/flox/Apoe–/– mice
Sample name: Macrophage-KO2
|
| Data processing |
The Applied Biosystems TaqMan Rodent miRNA A+B Cards Set v3.0 (catalog number 4444909) was used for this study. Taqman low density arrays were run on the 7900HT Fast Real-Time PCR System. The ABI TaqMan SDS v2.4 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRNAs from card A and B was used for analysis. Data were calculated using qBase software. Input data for analysis consisted of all samples. CT values were normalized to multiple endogenous controls (snoRNA202, snoRNA135 and MammU6 for Card A, snoRNA202, snoRNA135, MammU6 and snoRNA429 for Card B).
normalization: qBase-software computed signal (target gene signal normalized to endogenous controls and then scaled to WT group); Fold_Change: KO/WT
|
| |
|
| Submission date |
Oct 06, 2016 |
| Last update date |
May 23, 2018 |
| Contact name |
Andreas Schober |
| E-mail(s) |
aschober@med.lmu.de
|
| Organization name |
Ludwig-Maximilians-University
|
| Department |
Institute for Cardiovascular Prevention
|
| Street address |
Pettenkoferstr. 9
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18082 |
| Series (2) |
| GSE87718 |
miRNA expression profiles in Dicer WT and KO bone marrow-derived macrophages |
| GSE87721 |
Ago2-RIP-chip and miRNA expression profile in Dicer WT and KO macrophages from bone marrow or aorta |
|
