|
| Status |
Public on Aug 28, 2018 |
| Title |
Naïve-3h [EA12035_260028_MOGENE-1_0-ST-V1_3.3HNAIVE] |
| Sample type |
RNA |
| |
|
| Source name |
Bone-marrow derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
cells: Bone-marrow derived macrophages
group: Naïve
time point: 3h
genotype: H3.3-HA knock-in
|
| Treatment protocol |
Differentiated macrophages were treated with Interferon-gamma.
|
| Growth protocol |
Bone-marrow derived macrophages derived from H3.3-HA knockin mice were routinly generated by 10% of L-cell conditioned media in DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested from bone-marrow derived macrophages using Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
We obtained Microarray data (Affymetrix) from EA, A Quintiles Company. GeneSpring software was used for finding up/down regulated genes from the data (CHP files).
|
| |
|
| Submission date |
Oct 24, 2016 |
| Last update date |
Aug 30, 2018 |
| Contact name |
Rui Kamada |
| E-mail(s) |
kamadar@sci.hokudai.ac.jp
|
| Phone |
+81-11-706-2721
|
| Organization name |
Hokkaido University
|
| Department |
Department of Chemistry, Faculty of Science
|
| Lab |
Laboratory of Biological Science
|
| Street address |
N10 W8, Kita-ku
|
| City |
Sapporo |
| ZIP/Postal code |
060-0810 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE89092 |
Microarray analysis of interferon stimulated bone-marrow derived macrophages Transcriptomes |
| GSE89440 |
Interferon stimulation creates chromatin marks to establish transcriptional memory |
|
