|
| Status |
Public on Dec 13, 2016 |
| Title |
Malignant glioma clone U3118MG-C688 |
| Sample type |
genomic |
| |
|
| Source name |
Malignant glioma clone U3118MG-C688
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Malignant glioma clone
cell line: U3118MG-C688
|
| Growth protocol |
adherent cultures on laminin-coated dishes using neural stem cell media
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Dneasy blood and tissue kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
250 ng total genomic DNA was digested with the restriction enzyme Nsp I, then ligated to an Nsp I adaptor and further subjected to PCR amplification using a single primer. After fragmentation, the PCR products were labeled with a biotinylated nucleotide analogue using terminal deoxynucleotidyl transferase (TdT)
|
| |
|
| Hybridization protocol |
standard protocols for Affymetrix Cytoscan™ HD arrays (Affymetrix® Cytoscan User Guide (P/N 703038 Rev4.) Hybridized probes were captured by streptavidin-phycoerythrin conjugates using the GeneChip® Fluidics Station 450.
|
| Scan protocol |
The arrays were scanned using the GeneChip® Scanner 3000 7G
|
| Data processing |
Affymetrix® Chromosome Analysis Suite (ChAS) 2.0. Affymetrix-algorithm-name = CYTO2. Affymetrix-algorithm-version = 1.16.0. program-name = apt-copynumber-cyto.exe
Affymetrix CytoScan HD array of 30 clonal malignant glioma cultures and 6 bulk cultures representing the parental material used to derive clones.
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| |
|
| Submission date |
Nov 01, 2016 |
| Last update date |
Dec 13, 2016 |
| Contact name |
Bo Segerman |
| E-mail(s) |
bo.segerman@igp.uu.se
|
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskjolds v 20
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE89398 |
Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition (CytoScanHD) |
| GSE89401 |
Clonal variation in drug and radiation response among glioma-initiating cells is linked to proneural-mesenchymal transition |
|
