|
| Status |
Public on Dec 06, 2016 |
| Title |
embryo as control mRNA, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Zebrafish (Danio rerio) embryos
|
| Organism |
Danio rerio |
| Characteristics |
strain: Ablino embryos
|
| Treatment protocol |
Newly fertilized zebrafish embryos were treated with PM2.5 for 6-120 hpf, and dead embryos and larvae were removed every day. The dosage without cause mortality was 100 μg/mL of PM2.5 for following microarray analysis.
|
| Growth protocol |
Zebrafish embryos treated with PM2.5 for 6-120 hpf
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
embryo as control for 6-120 hpf
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 05, 2016 |
| Last update date |
Dec 06, 2016 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE90875 |
Expression data from zebrafish embryos |
|
