|
| Status |
Public on Mar 01, 2017 |
| Title |
LSK cultured 18h without EV rep4 |
| Sample type |
RNA |
| |
|
| Source name |
LSK cultured 18h without EV
|
| Organism |
Mus musculus |
| Characteristics |
cell type: HSPC
growth condition: 18h without EV
|
| Treatment protocol |
Cultured LSK cells were treated or not with Evs derived from AFT cells
|
| Growth protocol |
LSK cells recovered after cell corting or mainatin 18h in Myelocult Medium M5300
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Nucleospin miRNAeasy kit (macherey Nagel)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500pg total RNA (Mouse Gene 2.0, picoWT, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C and 60rpm on Gene Mouse2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G.
|
| Description |
Gene expression data from LSK cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 25, 2017 |
| Last update date |
Mar 02, 2017 |
| Contact name |
Gregoire Stik |
| E-mail(s) |
gregoire.stik@gmail.com
|
| Organization name |
CRG
|
| Street address |
88 Dr Aiguader
|
| City |
Barcelona |
| State/province |
select a state |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE94074 |
Expression data of Hematopoietic progenitor and stem cells after 18h of culture with or without extracellular vesicles secreted by AFT stromal cells |
|
