|
| Status |
Public on Dec 12, 2007 |
| Title |
Cortex_Control_rep1 (05SE1755) |
| Sample type |
RNA |
| |
|
| Source name |
FRONTOPARIETAL BRAIN CORTEX OF SHAM-OPERATED RATS
|
| Organism |
Rattus norvegicus |
| Characteristics |
Male Sprague Dawley rats (225-250 g) aged approximately two months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Reagent Protocol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix IVT Labeling kit
|
| |
|
| Hybridization protocol |
Samples were hybridised to Affymetrix RAE230 2.0 genechip using Affymetrix standard hybridization protocol using 15ug of biotinylated cRNA (Affymetrix Expression analysis technical manual). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
The chips were scanned using an Agilent GeneArray Scanner 2500
|
| Description |
comparison of olfactory-bulbectomized and sham-operated rats with depressive-like behaviour
|
| Data processing |
Feature intensity values from scanned arrays were normalised and reduced to expression summaries using RMA method and further normalised to the median of each probe
|
| |
|
| Submission date |
Dec 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Leyre Uriguen |
| E-mail(s) |
leyre.uriguen@ehu.es
|
| Phone |
+34946015674
|
| Fax |
+34946013220
|
| Organization name |
University of the Basque Country
|
| Department |
Pharmacology
|
| Street address |
Barrio Sarriena s/n
|
| City |
Leioa |
| State/province |
Vizcaya |
| ZIP/Postal code |
48940 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE9789 |
Gene expression analysis of brain cortex of olfactory-bulbectomized rats |
| GSE9798 |
Gene expression analysis of brain cortex |
|
