|
| Status |
Public on Feb 28, 2017 |
| Title |
Hippocampal T6 |
| Sample type |
RNA |
| |
|
| Source name |
POCD hippocampal tissue 6
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6
age: 12-14months
|
| Treatment protocol |
Mice from the POCD group were experienced tibia fracture surgery, mice from the control group did not receive any prior treatment or surgery.
|
| Growth protocol |
The animals used in this study were C57BL/6 mice purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The mice were male, aged 12–14 months, and weighed 25–35 g. The room temperature and humidity were controlled at 25°C and 55%, respectively. All mice were acclimated to the environment for 1 week prior to the initiation of the study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the POCD and control group hippocampal tissues using TRIzol (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions. Subsequently, total RNA was assessed by electrophoresis on a denaturing agarose gel and quantified by NanoDrop spectrophotometer (NanoDrop, USA).
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
Bilateral hippocampal tissue
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Feb 27, 2017 |
| Last update date |
Feb 28, 2017 |
| Contact name |
changwei wei |
| E-mail(s) |
wcw0025@sina.com
|
| Organization name |
Beijing Chao-Yang Hospital, Capital Medical University
|
| Department |
Department of Anesthesiology
|
| Street address |
8 Gongren Tiyuchang Nanlu, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100020 |
| Country |
China |
| |
|
| Platform ID |
GPL22782 |
| Series (1) |
| GSE95426 |
Comprehensive analysis of differentially expressed lncRNAs and mRNAs in aged mice |
|
