|
| Status |
Public on Aug 15, 2017 |
| Title |
GpatHF-HF195_D05 |
| Sample type |
RNA |
| |
|
| Source name |
Rat EDL muscle - Grandpaternal HFD on HFD
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: Female
tissue: Extensor Digitorum longus (EDL) muscle
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Sprague-Dawley female rats were maintaned under a temperature-controlled environment and 12:12-h light:dark cycle. Adult females were fed either a control chow diet or a high-fat diet (42/45% energy from fat) for 12 weeks. EDL muscle tissue was dissect and store at -80C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit from Qiagen according to manufacturer’s instructions. RNA quality was confirmed using the Agilent 2100 Bioanalyzer (Agilent Technologies), and RNA concentration was measured on a NanoDrop (Thermo Scientific).
|
| Label |
Biotin
|
| Label protocol |
200 ng of total RNA was used as input to generate sense-strand cDNA with Ambion® WT Expression kit (Life Technologies: PN 4425209). The generated sense-strand cDNA was fragmented and biotin labelled using Affymetrix® GeneChip WT Terminal Labeling Kit (Affymetrix: PN 900671).
|
| |
|
| Hybridization protocol |
Affymetrix GeneTitan instruments were used for hybridization, wash, stain and scanning of Rat Gene 1.1 ST Array Plates
|
| Scan protocol |
Arrays were scanned in a Affymetrix GeneTitan Scanner
|
| Data processing |
Generated CEL files were preprocessed using Affymetrix Expression Console using the Probe Logarithmic Intensity Error (PLIER) method for summarization, PM-GCBG for background correction and Global Median for normalization.
|
| |
|
| Submission date |
Feb 28, 2017 |
| Last update date |
Aug 15, 2017 |
| Contact name |
Thais de Castro Barbosa |
| Organization name |
Karolinska Institutet
|
| Department |
Physiology and Pharmacology
|
| Lab |
Integrative Physiology
|
| Street address |
von Eulers väg 4a
|
| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 77 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE95490 |
mRNA expression in Extensor Digitorum longus (EDL) muscle of adult F2 female offspring from F0-fathers fed a chow or high-fat diet |
|
