|
| Status |
Public on Feb 14, 2018 |
| Title |
LPA DL Le 2887 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Sus scrofa |
| Characteristics |
age: adult
body weight: 88.8
meat quality grade: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen miReasy Mini kit TRI Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from miRNA according to FlashTag Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix GeneChip miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
microRNA expression data from liver
|
| Data processing |
Data were analysed with Expression Console and quantify by med polish
|
| |
|
| Submission date |
Mar 31, 2017 |
| Last update date |
Feb 14, 2018 |
| Contact name |
Siriluck Ponsuksili |
| E-mail(s) |
s.wimmers@fbn-dummerstorf.de
|
| Organization name |
FBN
|
| Street address |
Wilhelm-stahl-allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE97274 |
Gene expression data of microRNA from liver of pig breed German Landrace |
|
