|
| Status |
Public on Jan 06, 2009 |
| Title |
large airways, smoker 161 |
| Sample type |
RNA |
| |
|
| Source name |
large airway epithelial cells obtained by bronchoscopy and brushing
|
| Organism |
Homo sapiens |
| Characteristics |
Age: 35
Sex: M
Ethnic group: black
Smoking status: smoker, 5 pack-years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA for HuGeneFL and HG-U133A or 2 microg total RNA for HG-U133 Plus 2.0 (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL, HG-U133A or HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneArray 2500 for HuGeneFL and the GeneChip Scanner 3000 7G for HG-U133A and HG-U133 Plus 2.0.
|
| Description |
Comparison of gene expression in large airway epithleium of normal non-smokers and normal smokers
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 10, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (6)
|
| GSE10135 |
Cigarette Smoking Induces Overexpression of a Fat Depleting Gene AZGP1 in the Human Airway Epithelium |
| GSE11906 |
Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium |
| GSE16696 |
Smoking is Associated with Shortened Airway Cilia |
| GSE17905 |
Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium |
| GSE18385 |
Smoking-induced Up-regulation of AKR1B10 Expression in the Airway Epithelium of Healthy Individuals |
| GSE19722 |
Airway Basal Cells of Healthy Smokers Express an Embryonic Stem Cell Signature Relevant to Lung Cancer |
|
| Relations |
| Reanalyzed by |
GSE119087 |
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