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Status |
Public on Jan 18, 2022 |
Title |
Telencephalon_nostril_injury_1dpl_rep3 |
Sample type |
RNA |
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Source name |
Telencephalon, nostril injury, 1 day post lesion
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Organism |
Danio rerio |
Characteristics |
age: 3-5 months genotype: wild-type
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Treatment protocol |
As described in the paper. We carried out different stab wound injury paradigms: nostril and skull injury. Fish were anesthetized with 0.02% MS222 (Sigma-Aldrich) and injuries were performed in both telencephalic hemispheres. Nostril injury was performed as described previously (Baumgart et al., 2012). For the skull injury, a micro-knife (Fine Science Tools) was inserted vertically through the skull into the medial region of the telencephalon.
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Growth protocol |
Fish were kept under standard husbandry conditions
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Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Kit (Qiagen) including on-column DNAse digestion
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Label |
biotin
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Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen)
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|
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Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
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Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
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Description |
Gene expression data from zebrafish telencephalon 1 day after nostril injury
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Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.3) including median polish and Sketch-Quantile normalisation
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Submission date |
Apr 26, 2017 |
Last update date |
Jan 18, 2022 |
Contact name |
Johannes Beckers |
E-mail(s) |
johannes.beckers@helmholtz-munich.de
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Organization name |
Helmholtz Zentrum Muenchen
|
Department |
Institute of Experimental Genetics
|
Street address |
Ingolstaedter Landstr. 1
|
City |
Neuherberg |
ZIP/Postal code |
85764 |
Country |
Germany |
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|
Platform ID |
GPL16933 |
Series (1) |
GSE98217 |
Activation of innate immune pathways after brain injury promotes scar formation and impairs tissue recovery |
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