|
| Status |
Public on Jan 18, 2022 |
| Title |
Telencephalon_skull_injury_1dpl_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Telencephalon, skull injury, 1 day post lesion
|
| Organism |
Danio rerio |
| Characteristics |
age: 3-5 months
genotype: wild-type
|
| Treatment protocol |
As described in the paper. We carried out different stab wound injury paradigms: nostril and skull injury. Fish were anesthetized with 0.02% MS222 (Sigma-Aldrich) and injuries were performed in both telencephalic hemispheres. Nostril injury was performed as described previously (Baumgart et al., 2012). For the skull injury, a micro-knife (Fine Science Tools) was inserted vertically through the skull into the medial region of the telencephalon.
|
| Growth protocol |
Fish were kept under standard husbandry conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit (Qiagen) including on-column DNAse digestion
|
| Label |
biotin
|
| Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
|
| Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
|
| Description |
Gene expression data from zebrafish telencephalon 1 day after skull injury
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.3) including median polish and Sketch-Quantile normalisation
|
| |
|
| Submission date |
Apr 26, 2017 |
| Last update date |
Jan 18, 2022 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE98217 |
Activation of innate immune pathways after brain injury promotes scar formation and impairs tissue recovery |
|
