|
| Status |
Public on Dec 28, 2008 |
| Title |
Muscle spaceflight 9 |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius, spaceflight, 11 days1 9hr
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6J; Gender: female; Age: 77 days
|
| Treatment protocol |
tissues were dissected and then snap frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
|
| Description |
AEM animals were housed at Cape Canaveral Air Force Station’s Hangar L orbital environmental simulator for 11 days and 19 h with conditions mimicking the shuttle’s middeck temperature, humidity, and CO2 levels
|
| Data processing |
Expression values were calculated using RMA; detection calls were calculated using MAS5
|
| |
|
| Submission date |
Feb 14, 2008 |
| Last update date |
Dec 28, 2008 |
| Contact name |
Jeremy Barth |
| E-mail(s) |
barthj@musc.edu
|
| Organization name |
MUSC
|
| Street address |
173 Ashley Ave, BSB 627
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425-5080 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE10533 |
Effects of spaceflight on murine skeletal muscle gene expression |
|
