|
| Status |
Public on Jun 30, 2017 |
| Title |
Non-pregnant islets #2 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic islets, non-pregnant mice
|
| Organism |
Mus |
| Characteristics |
strain: C57BL/6
gender: Female
age: 6-8 weeks
gestational status: non-pregnant
tissue: Pooled pancreatic islets
|
| Treatment protocol |
Mouse islets were isolated from non-pregnant mice or dams at gestational day 14.5. Islets from 3-4 mice were pooled to collect at least 500 islets per sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Immediately after isolation, total islet RNA was extracted with Stratagene RNA kit (Life Technologies).
|
| Label |
Cy3
|
| Label protocol |
SuperScript® Indirect RNA Amplification System with Alexa Fluor® reactive dye decapacks
|
| |
|
| Hybridization protocol |
Fragmented and labeled cDNA probes were hybridized with Invitrogen™ NCode™ non-coding RNA arrays
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C).
|
| Description |
Ctrl_2
|
| Data processing |
Signal intensity was normalized for expression analysis. Primer sequences were BLASTed against a database generated from the transcript sequences of all transcripts in the ab initio annotation. Only hits that were unique and perfect (60 nt at 100% match) were considered valid.
|
| |
|
| Submission date |
Jun 29, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Alex-Xianghua Zhou |
| E-mail(s) |
ALEXZHOU.SE@GMAIL.COM
|
| Phone |
0046739313297
|
| Organization name |
AstraZeneca R&D Gothenburg
|
| Street address |
Pepparedsleden 1
|
| City |
Mölndal |
| State/province |
- If in U.S, Select a State - |
| ZIP/Postal code |
43183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL20668 |
| Series (1) |
| GSE100645 |
Differentially expressed non-coding RNAs in mouse islets during pregnancy |
|
