|
| Status |
Public on Oct 11, 2018 |
| Title |
Dorsal subventricular zone, day 7 post lesion, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Dorsal subventricular zone from zebrafish, 7 days after stab-wound injury
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Dorsal subventricular zone
age: 3-6 months
genotype: Tg(gfap:gfp)mi2001 transgenic line
|
| Treatment protocol |
Cerebroventricular microinjections (CMVI) in the telencephalic region of the brain were performed as described (Kizil and Brand, 2011, Barbosa et al., 2015). Fish were anaesthetized in 0.02% MS222 and held in a sponge. A small hole in the skull was made in the region between the telencephalon and the optic tectum using a micro-knife (Fine Science Tools). After the injections, fish were woken up in the aerated tank water and kept in their normal husbandry conditions.
|
| Growth protocol |
Normal husbandry conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Micro Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
|
| Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
|
| Description |
Gene expression data from dorsal subventricular zone from zebrafish, 7 days after stab-wound injury
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.2) including median polish and Sketch-Quantile normalisation
|
| |
|
| Submission date |
Aug 09, 2017 |
| Last update date |
Oct 11, 2018 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE102400 |
The aryl hydrocarbon receptor pathway defines the time frame for restorative neurogenesis |
|
