|
| Status |
Public on May 31, 2018 |
| Title |
MEFdKO_AICAR_4h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MEFdKO, AICAR, 4h
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Murine Embryonic Fibroblasts
genotype: Ampk{alpha}1/{alpha}2 double knockout
culture: SV40-immortalized
agent: AICAR
time: 4h
|
| Treatment protocol |
Cells were stimulated at different times with 1 mM AICAR.
|
| Growth protocol |
MEFs were grown at low density in 10 cm dishes at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (Qiagen). The quality of samples was assessed on a Bioanalyzer 2100 (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
cRNAs were hybridized to Mouse MG-430 PM Array Strip Kit.
|
| Scan protocol |
Fluorescence intensities were quantified and analyzed using the Expression Console v 1.2 software.
|
| Description |
Gene expression data from cells at (t=4h) in the presence of 1mM AICAR
|
| Data processing |
The digital intensity values were then converted into cell intensity files using the Affymetrix GeneAtlas Software. Initial cut-offs for differential expression were set to a fold change threshold of ± 2 or above and a maximum adjusted p-value of 0.05.
|
| |
|
| Submission date |
Nov 02, 2017 |
| Last update date |
May 31, 2018 |
| Contact name |
Michel Moenner |
| E-mail(s) |
michel.moenner@u-bordeaux.fr
|
| Phone |
+33 688193836
|
| Organization name |
CNRS
|
| Department |
IBGC CNRS UMR5095
|
| Lab |
Genetics of Metabolic Pathways
|
| Street address |
1, rue Camille Saint-Saens
|
| City |
Bordeaux |
| ZIP/Postal code |
33077 |
| Country |
France |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE106460 |
Expression data from AMPKalpha1/2-double knockout murine embryonic fibroblasts stimulated or not with AICAR |
|
