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| Status |
Public on May 31, 2018 |
| Title |
Expression data from AMPKalpha1/2-double knockout murine embryonic fibroblasts stimulated or not with AICAR |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
AICAR is a metabolite with anti-tumoral properties. Its monophosphate derivative ZMP is an AMP mimetic activator of the protein kinases AMPK. However, AICAR also mediates AMPK-independent effects.
A kinetic transcriptome analysis was therefore carried out in Ampkα1/α2 double knockout murine embryonic fibroblasts in response to AICAR in order to unveil these AMPK-independent functions,
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| Overall design |
Ampkα1/α2 double knockout murine embryonic cells were incubated with 1 mM AICAR for 0, 1, 2, 4, 8, 12 and 24h. RNA extraction and hybridization on Affymetrix (Mouse MG-430 PM Array Strip Kit) were then performed. Analyses were performed in triplicates (except t=8h in duplicate). The fold change in gene expression corresponds to the ratio between each incubation times with AICAR and control (t=0) without AICAR.
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| Contributor(s) |
Philippe C, Pinson B, Dompierre J, Pantesco V, Viollet B, Daignan-Fornier B, Moenner M |
| Citation(s) |
29730476 |
| Submission date |
Nov 02, 2017 |
| Last update date |
Aug 30, 2018 |
| Contact name |
Michel Moenner |
| E-mail(s) |
michel.moenner@u-bordeaux.fr
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| Phone |
+33 688193836
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| Organization name |
CNRS
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| Department |
IBGC CNRS UMR5095
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| Lab |
Genetics of Metabolic Pathways
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| Street address |
1, rue Camille Saint-Saens
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| City |
Bordeaux |
| ZIP/Postal code |
33077 |
| Country |
France |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA416925 |
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