|
| Status |
Public on Jul 13, 2009 |
| Title |
small airways, smoker 130 |
| Sample type |
RNA |
| |
|
| Source name |
airway epithelial cells obtained by bronchoscopy and brushing
|
| Organism |
Homo sapiens |
| Characteristics |
age: 41
sex: M
ethnic group: white
smoking status: smoker, 12 pack-years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
none
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 13, 2008 |
| Last update date |
Nov 12, 2009 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (10)
|
| GSE11784 |
Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking |
| GSE11906 |
Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium |
| GSE11952 |
Coordinate Control of Nrf2 Mediated Genes in the Human Small Airway Epithelium Highly Responsive to Smoking |
| GSE13931 |
Disparate Oxidant-related Gene Expression of Human Small Airway Epithelium Compared to Autologous Alveolar Macrophages |
| GSE17905 |
Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium |
| GSE18385 |
Smoking-induced Up-regulation of AKR1B10 Expression in the Airway Epithelium of Healthy Individuals |
| GSE19407 |
Smoking-induced Wnt pathway downregulation |
| GSE19667 |
Threshold of Biologic Response of the Small Airway Epithelium to Low Levels of Tobacco Smoke |
| GSE20250 |
CNV Polymorphisms Modulate the Expression of GST Subtypes in the Human Small Airway Epithelium and Alveolar Macrophages |
| GSE20257 |
Smoking-induced Disarray of the Apical Junctional Complex Gene Expression Architecture in the Human Airway Epithelium |
|
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