|
| Status |
Public on Sep 01, 2009 |
| Title |
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
| Treatment protocol |
Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
|
| Growth protocol |
Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
|
| |
|
| Hybridization protocol |
25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
|
| Description |
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep2
|
| Data processing |
The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
|
| |
|
| Submission date |
Jun 17, 2008 |
| Last update date |
Aug 10, 2012 |
| Contact name |
John Zagorski |
| E-mail(s) |
john.zagorski@carolinas.org, j_zagorski@hotmail.com
|
| Phone |
704-355-3663
|
| Organization name |
Carolinas Medical Center
|
| Department |
Emergency Medicine
|
| Lab |
Cannon Research Center
|
| Street address |
1542 Garden Terrace, 301
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11851 |
Gene expression in rat right ventricles during chronic pulmonary embolism |
|
