|
Status |
Public on Jul 06, 2009 |
Title |
24 hrs after the third OVA challenge Lung tissue Group3_5 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Placebo treated control pool (Cy3)
|
Organism |
Mus musculus |
Characteristics |
Balb/c mice placebo challenged control lung tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy kit Quality controlled by Agilent bioanalyzer
|
Label |
Cy3
|
Label protocol |
Agilent dual color microarray protocol version 5.5 Data normalisation by Agilent Feature Extraction 7.5
|
|
|
Channel 2 |
Source name |
OVA challenged mice
|
Organism |
Mus musculus |
Characteristics |
Balb/c mice treated and challenged with OVA lung tissue RNA Group3: 24 hours after the third OVA allergen challenge (day31), after the AHR measurement
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Qiagen RNeasy kit Quality controlled by Agilent bioanalyzer
|
Label |
Cy5
|
Label protocol |
Agilent dual color microarray protocol version 5.5 Data normalisation by Agilent Feature Extraction 7.5
|
|
|
|
Hybridization protocol |
Agilent dual color protocol version 5.5
|
Scan protocol |
Agilent microarray scanner default setting for 4x44k dual color microarray
|
Description |
This is the fifth sample of the four biological replicates, belonging to group3
|
Data processing |
Data were normalised by Agilent Feature Extraction 7.5 Value is normalized log10Ratio, default Per Spot and Per Chip: Intensity dependent (Lowess) normalization
|
|
|
Submission date |
Jun 27, 2008 |
Last update date |
Jul 06, 2009 |
Contact name |
Gergely Tolgyesi |
E-mail(s) |
tolgyesi_gergely@yahoo.com
|
Organization name |
Semmelweis University Budapest
|
Street address |
Nagyvarad ter 4
|
City |
Budapest |
ZIP/Postal code |
1089 |
Country |
Hungary |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE11911 |
Gene expression profiling of experimental asthma reveals a possible role of paraoxonase-1 in asthma |
|