|
| Status |
Public on Jul 18, 2008 |
| Title |
Highly metastatic 468GFP-LN biorep2 |
| Sample type |
genomic |
| |
|
| Source name |
Parental Cell line 468GFP-LN, Second biological replicate
|
| Organism |
Homo sapiens |
| Characteristics |
Parental Cell line 468GFP-LN, Second biological replicate
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Cells were grown out to three passages in alpha-MEM containing 10% FBS in 5% CO2 to 70 % confluency
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Sigma GeneElute kit using the Manufacturer's protocol
|
| Label |
Biotin/SAPE
|
| Label protocol |
Ligation mediated PCR amplified genomic DNA, after restriction with HhaI, was labeled as per Affymetrix standard protocol for Human Promoter 1.0R arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 micrograms of Ligation mediated PCR amplified genomic DNA was hybridized as per standard affymetrix protocols for Human Promoter 1.0R arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Ligation mediated PCR amplified genomic DNA, after restriction with HhaI
Promoter array data from Parental Cell line 468GFP-LN, Second biological replicate
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 15, 2008 |
| Last update date |
Jul 17, 2008 |
| Contact name |
Ann Chambers |
| E-mail(s) |
joseph.andrews@lhsc.on.ca
|
| Phone |
(519) 685-8430
|
| Organization name |
London Regional Cancer program
|
| Department |
Victoria research labs
|
| Street address |
790 Commissioner's Road E
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL5082 |
| Series (2) |
| GSE12122 |
Epigenetic mapping and functional analysis in a breast cancer metastasis model using whole genome tiling arrays |
| GSE15619 |
Epigenetic signature of breast cancer and its association with gene expression and copy number |
|
