|
| Status |
Public on Sep 27, 2018 |
| Title |
PRMT1-cKO2 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: PRMT1-cKO
tissue: Heart
age: 6 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cardiac total RNA was extracted using ISOGEN II (Nippon Gene, #311-07361) according to manufacturer's instruction. Ribosomal RNA (rRNA) was removed using RiboMinus Eukaryote Kit (Thermo Fisher Scientific, #A1083708).
cDNA libraries were prepared using SOLiD total RNA-Seq kit (Thermo Fisher Scientific, #4445374) according to manufacturer's instruction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB 5500xl Genetic Analyzer |
| |
|
| Description |
processed data file: PRMT1-WT_vs_PRMT1-CKO.xlsx
|
| Data processing |
Reads were mapped on GRCm38 mouse reference genome and quantified using CLC Genomics Workbench (Qiagen).
Read counts were normalized by calculating number of reads per kilobase per million (RPKM) for each transcript in individual samples using the CLC Genomic Workbench software (Qiagen).
Genome_build: GRCm38
Supplementary_files_format_and_content: PRMT1-WT_vs_PRMT1-CKO.xlsx: Excel file
|
| |
|
| Submission date |
Apr 10, 2018 |
| Last update date |
Sep 27, 2018 |
| Contact name |
Jun-Dal Kim |
| Organization name |
University of Toyama
|
| Department |
Department of Complex Biosystem Research (CBR), Institute of National Medicine (INM)
|
| Lab |
Complex Biosystem Research Lab.
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15907 |
| Series (1) |
| GSE112938 |
RNA-seq analysis of hearts from control and cardiomyocyte-specific PRMT1-deficient mice |
|
| Relations |
| BioSample |
SAMN08906293 |
