|
Status |
Public on Feb 28, 2009 |
Title |
AGTR2 knockout replicate 1 dye-swap |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
whole brain E15 Knockout
|
Organism |
Mus musculus |
Characteristics |
C57BL/6 x 129;male;E15
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from frozen slices of whole brain using the Qiagen Rneasy kit according to manufacturer instructions followed by Dnase with the Ambion Turbo Dnase-free kit
|
Label |
Cy3
|
Label protocol |
Agilent low RNA input linear amplification kit with Cy3 or Cy5-CTP according to maunfacturer instructions
|
|
|
Channel 2 |
Source name |
whole brain E15 control replicate one
|
Organism |
Mus musculus |
Characteristics |
C57BL/6 x 129;male;E15
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from frozen slices of whole brain using the Qiagen Rneasy kit according to manufacturer instructions followed by Dnase with the Ambion Turbo Dnase-free kit
|
Label |
Cy5
|
Label protocol |
Agilent low RNA input linear amplification kit with Cy3 or Cy5-CTP according to maunfacturer instructions
|
|
|
|
Hybridization protocol |
Agilent hybridization protocol for 17 hours at 65 degrees Celcius
|
Scan protocol |
Agilent scanner G2505B, 10 micron resolution extracted with Agilent Feature Extraction v 8.0 software
|
Description |
none
|
Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
|
|
Submission date |
Aug 11, 2008 |
Last update date |
Dec 31, 2008 |
Contact name |
traci pawlowski |
E-mail(s) |
tpawlowski@tgen.org
|
Phone |
602-343-8574
|
Organization name |
Greenwood Genetic Ctr
|
Department |
research
|
Lab |
Srivastava
|
Street address |
125 Gregor Mendel Circle
|
City |
Greenwood |
State/province |
SC |
ZIP/Postal code |
29646 |
Country |
USA |
|
|
Platform ID |
GPL2872 |
Series (1) |
GSE12412 |
Embryonic day 15 and Postnatal day 1 whole mouse brain AGTR2 knockout versus control |
|