|
Status |
Public on Apr 20, 2018 |
Title |
030326 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Adipose Tissue of Type 2 Diabetic subject #5A before TZD treatment
|
Organism |
Homo sapiens |
Characteristics |
RNA_quality: null
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
|
|
Channel 2 |
Source name |
Adipose Tissue of Type 2 Diabetic subject #5C after TZD treatment
|
Organism |
Homo sapiens |
Characteristics |
RNA_quality: null
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy5
|
Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
|
|
|
Hybridization protocol |
Agilent : 3000.0 ng labelled nucleic acids hybridized at 62 degree_C during 17 hours
|
Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
Description |
Adipose Tissue of Type 2 Diabetic subject #5A before TZD treatment vs Adipose Tissue of Type 2 Diabetic subject #5C after TZD treatment Date:2007/01/26
|
Data processing |
Lowess method followed by block median normalization
|
|
|
Submission date |
Sep 16, 2008 |
Last update date |
Apr 20, 2018 |
Contact name |
emmanuelle meugnier |
E-mail(s) |
meugnier@univ-lyon1.fr
|
Organization name |
INSERMU1060/INRA1397
|
Lab |
CarMeN
|
Street address |
Cens Eli Bat 2 D- Hopital Lyon Sud
|
City |
Pierre Bénite |
ZIP/Postal code |
69495 |
Country |
France |
|
|
Platform ID |
GPL1456 |
Series (2) |
GSE12793 |
Analysis of Rosiglitazone therapy on adipose tissue in type 2 diabetic patients |
GSE12895 |
Rosiglitazone effect on type 2 diabetic patients: adipose tissue |
|