|
| Status |
Public on Nov 19, 2018 |
| Title |
MEF_105 |
| Sample type |
SRA |
| |
|
| Source name |
mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: embryonic fibroblasts
passages: 1-2
treatment: growth medium
time: 2d
|
| Treatment protocol |
Ci-Ics: MEFs treated by chemical cocktails (VCRTs) under hypoxia condition (5% O2) for 6 days. Ci-chons: chondrogensis induction of ci-Ics for 28 days.
|
| Growth protocol |
Before treatment, MEFs were cultured in 10% FBS+ H-DMEM at a confluency of 90%. Mchons were cultured in 10% FBS + DMEM/F12 a confluency of 80-90%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single cells were lysated, preamplified and library prepared according C1-HT IFC protocol of Fluidigm Corporation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
WBJPE174099582
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
alignment was conducted with Tophat
The final gene expression data was normalized using Cuffnorm and represented as counts
Genome_build: mm9
Supplementary_files_format_and_content: Csv file includes raw counts of each gene from each sample
|
| |
|
| Submission date |
Aug 07, 2018 |
| Last update date |
Nov 19, 2018 |
| Contact name |
Yishan Chen |
| E-mail(s) |
YishanChen@intl.zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
#866 Yuhangtang Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE118234 |
Single cell dissection of Chemical Reprogramming from Fibroblasts into functional Chondrocytes |
| GSE118236 |
High-Resolution Dissection of Chemical Reprogramming from Fibroblasts into Functional Articular Chondrocytes |
|
| Relations |
| BioSample |
SAMN09777684 |
| SRA |
SRX4516845 |
