|
| Status |
Public on Dec 10, 2018 |
| Title |
BT474.Entinostat.C |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human Reference B
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Human Reference B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated by stratagene
|
| Label |
Cy3
|
| Label protocol |
described in Hu et al., 2005, BioTechniques, 38:121-124
|
| |
|
| Channel 2 |
| Source name |
Breast cancer, Cell_lines, Entinostat-treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BT474
treatment: Treated by Entinostat for 72 hours at the IC50 doses
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated by stratagene
|
| Label |
Cy5
|
| Label protocol |
described in Hu et al., 2005, BioTechniques, 38:121-124
|
| |
|
| |
| Hybridization protocol |
described in Hu et al., 2005, BioTechniques, 38:121-124
|
| Scan protocol |
Agilent Technologies Scanner G2505C US82800149 Using Feature Extraction Software
|
| Description |
cell line
|
| Data processing |
Data for both channels were Lowess-normalized and then log(2) ratio was taken
|
| |
|
| Submission date |
Aug 19, 2018 |
| Last update date |
Dec 10, 2018 |
| Contact name |
Charles M. Perou |
| E-mail(s) |
cperou@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Professor of Genetics, and Pathology & Laboratory Medicine; Lineberger Comprehensive Cancer Center
|
| Street address |
12-044 Lineberger Comprehensive Cancer Center CB# 7295
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platform ID |
GPL10481 |
| Series (2) |
| GSE118741 |
Identification of Jun loss that promotes resistance to HDAC inhibitor Entinostat through Myc signaling in Luminal breast cancer [cell line] |
| GSE118744 |
Identification of Jun loss that promotes resistance to HDAC inhibitor Entinostat through Myc signaling in Luminal breast cancer |
|
