|
| Status |
Public on Jan 20, 2021 |
| Title |
5 days after treatment, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
hearts from zebrafish at 5 days after 5-weeks of PHZ treatment
|
| Organism |
Danio rerio |
| Characteristics |
treatment: at 5 days after 5-weeks of PHZ treatment
tissue: Heart
|
| Treatment protocol |
PHZ was added to the fish water of the treated group for 30 min every 3 days for 5 weeks. The 30 min incubation with PHZ was followed by a 30 min wash in fish water. PHZ was used at a concentration of 1.25 μg/mL the first week followed by 2.5 μg/mL for the rest of the protocol. The control group followed the same process without the addition of PHZ in the fish water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 3 biological replicates each containing 3 ventricles were isolated per time point using the RNeasy® micro kit (Qiagen). Potential contaminating genomic DNA was digested by DNase I treatment (Qiagen).
|
| Label |
biotin
|
| Label protocol |
250 ng of total RNAs were reverse transcribed into cDNA, then transcribed into cRNAs and labeled into biotinylated cRNA using the GeneChip WT Reagent kit (Affymetrix) according to the manufacturer’s standard protocols
|
| |
|
| Hybridization protocol |
Arrays were hybridized, washed and stained using the Affymetrix GeneChip WT Terminal Labeling and Hybridization kit.
|
| Scan protocol |
GeneChip Scanner 3000 according to the manufacturer’s standard procedure
|
| Description |
Gene expression data from the heart of zebrafish at 5 days after 5-weeks of PHZ treatment
|
| Data processing |
Microarray data were pre-processed by Oligo R package using the robust multi-chip analysis (RMA)
|
| |
|
| Submission date |
Oct 09, 2018 |
| Last update date |
Jan 20, 2021 |
| Contact name |
Yvan Devaux |
| E-mail(s) |
yvan.devaux@lih.lu
|
| Organization name |
LIH
|
| Lab |
Cardiovascular Research Unit
|
| Street address |
120 route d'Arlon
|
| City |
Luxembourg |
| ZIP/Postal code |
1150 |
| Country |
Luxembourg |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE120962 |
Gene expression data from the heart during regeneration in a zebrafish model of heart failure |
|
