|
| Status |
Public on Nov 01, 2018 |
| Title |
CRH5 sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
tumor from CRH5 cells
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
strain: BALB/c
|
| Treatment protocol |
Mice were not treated.
|
| Growth protocol |
BALB/c mice were injected subcutaneously with 100,000 of either CRH5 or EOH6 MM cells. When tumors reached 100 mm3 they were excised and total RNA extracted. At the same time, RNA was isolated from lungs and kidney excised from naïve BALB/c mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Clariom S Mouse Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
RNA expression in the different tissues was evaluated using the Clariom S Mouse Array (Affymetrix).
|
| Description |
Gene expression data
|
| Data processing |
Expression values were normalized and summarized into transcript clusters for analysis using Robust Multi-array Average approach in Array Studio (OmicSoft, Cary, NC).
|
| |
|
| Submission date |
Oct 31, 2018 |
| Last update date |
Nov 01, 2018 |
| Contact name |
Pietro Bertino |
| E-mail(s) |
pbertino@hawaii.edu
|
| Phone |
808 450 9618
|
| Organization name |
University of Hawaii
|
| Department |
Cell and Molecular Biology
|
| Street address |
651 Ilalo St., BSB222
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96813 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE122004 |
Expression data from mouse Malignant Mesothelioma (MM) tumors and normal tissues |
|
