|
| Status |
Public on Nov 14, 2018 |
| Title |
WT rep1 |
| Sample type |
SRA |
| |
|
| Source name |
fly head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
age: post eclosion day 3
genotype: 5905
Sex: female
|
| Growth protocol |
Flies were cultured in standard media at 25ºC with 60% humidity unless otherwise specified. The control (WT) line used was 5905 (FlyBase ID FBst0005905, w1118). The given genotype flies were collected at the day of eclosion and transferred to new vials every other day during aging process.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Heads were removed at the given age, RNA was harvested using Trizol reagent, and polyA tail RNAs were selected.
NEBNext Ultra RNA library Prep Kit for Illumina (NEB, E7530L) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
foxo_vs_5905_female_head.csv
|
| Data processing |
Sequencing reads were mapped to UCSC dm6(for sample 1-6) or flybase dm6_r6.05(for sample 7-18) with STAR by default parameter.
Read counts for each gene were calculated by HTSeq-count.
Count files were used as input to DESeq for normalization and differential expression analysis.
Genome_build: dm6
Supplementary_files_format_and_content: For sample1-6, differential expression between foxoc431/c431/WT was called using DESeq. For sample 7-18, sequencing depth was normalized to the same using DESeq.
|
| |
|
| Submission date |
Nov 13, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Xiaofen Wu |
| E-mail(s) |
xiaofenwu@sioc.ac.cn
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institute of Organic Chemistry
|
| Street address |
345 Lingling Rd
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN10414389 |
| SRA |
SRX5001529 |
