|
| Status |
Public on Dec 11, 2018 |
| Title |
WT, Control |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius muscle at 3 weeks after vehicle treatment of wild type
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
tissue: Gastrocnemius muscle tissue
agent: control
|
| Treatment protocol |
Intraperitoneally with sodium citrate (control) or STZ [50 mg/kg (Wako, Osaka, Japan), dissolved in 50 mM sodium citrate (pH 4.5)] on five consecutive days.
|
| Growth protocol |
Gastrocnemius muscle collections of mice at 3 weeks after vehicle or STZ treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse skeletal muscle with the use of the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
none
|
| Data processing |
rma-gene-full-Signal
|
| |
|
| Submission date |
Dec 10, 2018 |
| Last update date |
Dec 11, 2018 |
| Contact name |
Yu Hirata |
| E-mail(s) |
yuhirata@med.kobe-u.ac.jp
|
| Organization name |
Kobe University
|
| Street address |
chuo-ku, kusunoki-chou, 7-5-2
|
| City |
kobe |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE123540 |
Expression data from mouse skeletal muscle |
|
