|
| Status |
Public on Jan 19, 2019 |
| Title |
OMP--JeKo 48h |
| Sample type |
RNA |
| |
|
| Source name |
OMP--JeKo 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: jeKo
tissue: Notch1 wt MCL cell line
|
| Treatment protocol |
cells were stimulated with DLL4 4 ug/mL and treated with 25 ug/mL OMP-52M51
|
| Growth protocol |
cells culured with RPMI 1640 supplemented with 10-20 % FCS. 2 mM Glutamine And 50 ug/ml penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was done according manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according affymetrix protocols fron 150 ng total RNA
|
| |
|
| Hybridization protocol |
RNA samples were processed in Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the Gene Titan instrument.
|
| Description |
gene expression data in JeKo cells treated with OMP-52M51 48h
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
Raw data were normalized using the Robust Multichip Analysis (RMA) algorithm if the bioconductor Affy package
|
| |
|
| Submission date |
Jan 18, 2019 |
| Last update date |
Jan 19, 2019 |
| Contact name |
DOLORS COLOMER |
| E-mail(s) |
dcolomer@clinic.cat
|
| Phone |
34 660775688
|
| Organization name |
IDIBAPS-HOSPITAL CLINIC
|
| Street address |
VILLARROEL 170
|
| City |
BARCELONA |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE125349 |
MCL cel lines upon OMP-52M51 treatment |
|
